GF156

B18R, Human Recombinant Carrier-Free

B18R protein can be used with the Simplicon^® RNA Reprogramming Technology (Catalog No. SCR550) to create “foot-print” free human iPS cells at the highest efficiency without unwanted RNA degradation caused by cellular IFN responses.

• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

Propriedades

• fonte biológica: human
• Nível de qualidade: 100
• Ensaio: >98% (SDS-PAGE)
• forma: liquid
• peso molecular: M_w 38,389 Da
• concentração: 0.5 mg/mL
• técnica(s): cell culture | stem cell: suitable
• Impurezas: <0.05 ng/μg cytokine (LAL test)
• entrada: sample type induced pluripotent stem cell(s)
• Condições de expedição: dry ice

Descrição

Descrição geral

B18R is a virally-encoded protein that acts as a decoy receptor for Type 1 Interferons. B18R enables increased cell viability during RNA transfection protocols designed to convert human somatic donor cells into iPSCs via direct delivery of synthetic mRNA′s. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta.

Product Source: Insect cells infected with baculovirus: amino acids His20-Glu 351 with a His-tag attached of Vaccinia Virus B18R.

Aplicação

Research Category
Stem Cell Research

forma física

Sterile liquid;10 mM phosphate, 0.5 M NaCl, pH 7.5 buffer and is filtered with a 0.2µm filter before vialing.

Armazenamento e estabilidade

For best recovery, quick-spin vial prior to opening. Thaw on ice. While on ice, aliquot B18R protein into sterile, nuclease-free, low protein-binding eppendorf tubes and store at -80°C. Limit repeated freeze and thaw cycle. Use in a sterile environment.
Further dilutions should be made into appropriate buffer or medium.

Nota de análise

The DTT-reduced protein migrates as a 48 kDa polypeptide on SDS-PAGE due to glycosylation. The non-reduced protein migrates as a 46 kDa polypeptide on SDS-PAGE due to internal cystines.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable