ABT58

Anti-phospho PKA-RII (Ser96) Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): PRKAR2A, protein kinase, cAMP-dependent, regulatory, type II, alpha
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human, rat
• reatividade da espécie (prevista por homologia): mouse (based on 100% sequence homology)
• técnica(s): western blot: suitable
• nº de adesão NCBI: NP_032950
• nº de adesão UniProt: P12367
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: phosphorylation (pSer96)
• Informações sobre genes: human ... PRKAR2A(5576)

Descrição

Descrição geral

cAMP-dependent protein kinase type II-alpha regulatory subunit (PKA-RIIα) is an enzymatic protein belonging to the cAMP-dependent kinase regulatory chain family, a group of regulatory proteins involved in the PKA signaling pathway. This pathway has been shown to have regulatory roles in development, growth, and mortality of various organisms. PKA-RIIα is a subunit of the PKA type II regulatory chain, one of two PKA isoforms, and is involved in the regulation of membrane interactions through its binding to anchoring proteins, such as MAP2 kinase. Before its activation with cAMP, the enzyme is comprised of two catalytic chains and two regulatory chains, which split into two catalytic monomers and one regulatory dimmer after interacting with cAMP.

Especificidade

The antibody recognizes PKA-RIIalpha when phosphorylated at Ser96.

Imunogênio

Epitope: Phosphorylated Ser96 of PKA-RIIalpha

KLH-conjugated linear peptide corresponding to mouse PKA-RIIalpha phosphorylated at Ser96.

Aplicação

Research Category
Cell Structure

Signaling

Research Sub Category
Cytoskeletal Signaling

Kinases & Phosphatases

This Anti-phospho PKA-RII (Ser96) Antibody is validated for use in WB for the detection of phospho PKA-RII (Ser96).

Qualidade

Evaluated by Western Blot in Forskolin untreated and treated PC12 cell lysates.

Western Blot Analysis: 1 µg/mL of the antibody detected PKA-RIIalpha (Ser96) in 10 µg of Forskolin untreated and treated PC12 cell lysates.

Descrição-alvo

~ 51 kDa MW observed. The specificity of the target was shown by Lambda phosphatase treatment.

Ligação

Replaces: 06-704

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Forskolin untreated and treated PC12 cell lysates

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable