ABS1509

Anti-phospho-Neph1 (Tyr637/638) Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): Kin of IRRE-like protein 1, Kin of irregular chiasm-like protein 1, Nephrin-like protein 1, phospho-Neph1 (Tyr637/638)
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• reatividade da espécie (prevista por homologia): rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• técnica(s): western blot: suitable
• nº de adesão NCBI: NP_001164456
• nº de adesão UniProt: Q80W68
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: phosphorylation (pTyr637/pTyr638 )
• Informações sobre genes: human ... KIRREL1(55243)

Descrição

Descrição geral

Kin of IRRE-like protein 1 (UniProt Q80W68; also known as Nephrin-like protein 1, Kin of IRRE like 1, Kin of irregular chiasm-like protein 1) is encoded by the Kirrel (also known as 6720469N11Rik, Kirrel1, Neph1) gene (Gene ID 170643) in murine species. Podocytes are specialized epithelial cells that are critical components of the glomerular filtration barrier. Glomerular injury, such as that caused by renal ischemia, is often characterized by the effacement of podocytes, loss of slit diaphragms, and proteinuria. The podocyte proteins Neph1 and nephrin are critical for maintaining the structural integrity of slit diaphragm and therefore the filtration function of the glomerulus by organizing a signaling complex at the podocyte cell membrane. The cytoplasmic domain of Neph1 plays a key role in actin cytoskeleton remodeling events that are directly associated with Fyn-mediated Neph1 pTyr637/638 phosphorylation that leads to the recruitment of adapter proteins and signaling molecules, such as Grb2, Csk tyrosine kinase, and ZO-1. Phosphorylation-induced association between Neph1 and ZO-1 plays a key role in defining the tight junction formation in podocytes.

Especificidade

Mouse Neph1 Tyr637/638 site is equivalent to human Neph1 Tyr605/606. Equivalent target site is also present in human spliced isoforms 2&3 (Tyr621/622 & Tyr502/503, respectively), but not in murine spliced isoform 2.

Imunogênio

Epitope: Cytoplasmic domain

Linear peptide corresponding to the cytoplasmic domain of mouse phospho-Neph1 (Tyr637/638).

Aplicação

Detect Neph1 using this rabbit Polyclonal Anti-phospho-Neph1 Antibody (Tyr637/638), Cat. No. ABS1509, validated for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Western Blotting Analysis: A representative lot detected Neph1 phosphorylation in kinase assay using Fyn and COS-7 lysate containing exogenously expressed Neph1 (Data courtesy of Deepak Nihalini, University of Pennsylvania)
Western Blotting Analysis: A representative lot detected Neph1 phosphorylation in lysates from puromycin aminonucleoside-/PAN-treated human pocodytes (Arif, E., et al. (2014). J Biol Chem. 289(14):9502-9518).

Qualidade

Evaluated by Western Blotting in pervanadate treated human podocyte cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Neph1 phosphorylation at the target tyrosine residues in 7.5 µL of pervanadate treated human podocyte cell lysate.

Descrição-alvo

~120 kDa observed. Target band size appears larger than the calculated molecular weight (87.2 kDa/murine isoform 1, 72.4-85.0 kDa/human isoform 1-3) due to posttranslational modifications (glycosylation & phosphorylation). Uncharacterized band(s) may appear in some lysates.

forma física

Affinity purified

Purified rabbit polyclonal antibody in buffer containing PBS/glycine/5% BSA, .02% Sodium Azide and 30% glycerol.

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3