ABN51
Anti-Fox-3 Antibody
from rabbit, purified by affinity chromatography
Sinônimo(s):
RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Neuronal nuclei antigen, NeuN antigen
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
affinity isolated antibody
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
purificado por
affinity chromatography
reatividade de espécies
mouse, human, rat
técnica(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... RBFOX3(146713)
Descrição geral
RNA binding protein fox-1 homolog 3 (UniProt: Q8BIF2; also known as Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Fox-3, Neuronal nuclei antigen, NeuN antigen) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID: 52897) in murine species. Fox-3 acts as a pre-mRNA alternative splicing regulator. It regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD). It is widely expressed in brain, including in cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, and in the spinal cord (at protein level). However, Purkinje cells lack Fox-3 expression. It is also found in the retina in the ganglion cells and some cells in the inner nuclear layer, but is absent from the photoreceptor cells and most cells in the inner nuclear layer. In developing mouse it is expressed in the neural tube as early as day E9.5. Fox-3 expression is shown to be up-regulated by retinoic acid in p19 cells during neural differentiation. Knockout mice display significantly reduced brain weight, impaired neurofilament expression and decreased white matter volume, but normal total body mass. Five isoforms of Fox-3 have been described that are generated by alternative splicing.
Especificidade
This antibody recognizes Fox-3 at the N-terminus.
Imunogênio
Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of mouse Fox-3.
Aplicação
Anti-Fox-3, Cat. No. ABN51, is a highly specific rabbit polyclonal antibody that targets Fox 3/NeuN and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Western Blotting Analysis: 2 µg/mL from a representative lot detected Fox-3 in mouse brain nuclear extract.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Fox-3 in mouse cerebral cortex, mouse hippocampus, and human cerebellum tissues.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Fox-3 in E18 rat cortex cells.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Fox-3 in mouse cerebral cortex, mouse hippocampus, and human cerebellum tissues.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Fox-3 in E18 rat cortex cells.
Qualidade
Evaluated by Western Blot in mouse e16 brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Fox-3 on 10 µg of mouse e16 brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Fox-3 on 10 µg of mouse e16 brain tissue lysate.
Descrição-alvo
~46 kDa observed. An uncharacterized band appears at ~68 kDa in some lysates.
forma física
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Armazenamento e estabilidade
Stable for 1 year at 2-8°C from date of receipt.
Nota de análise
Control
Mouse e16 brain tissue lysate
Mouse e16 brain tissue lysate
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Preços
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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