ABN194

Anti-EVI-5 Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): Ecotropic viral integration site 5 protein homolog, EVI-5
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: mouse, rat
• reatividade da espécie (prevista por homologia): human (immunogen homology)
• técnica(s): immunohistochemistry: suitable; western blot: suitable
• nº de adesão NCBI: NP_005656
• nº de adesão UniProt: O60447
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... EVI5(7813)

Descrição

Descrição geral

Also known as Ecotropic viral integration site 5 protein homolog, EVI-5 serves as a key regulator of the cell cycle through time-dependent stabilization of Emi1, FBX05 protein, and APC/C ubiquitination substrates, such as cyclin A. EVI-5 accumulates in early G1 following Plk1 destruction, but is degraded in a Plk1- and ubiquitin-dependent manner in early mitosis. EVI-5 ablation causes premature APC/C activation, SCF(betaTrCP)-dependent Emi1 degradation, and cyclin destruction, all of which leads to cell cycle arrest and cytokinesis failure. It exists as a monomer and a dimer, and it may form complexes with alpha- and gamma-tubulin or interact with the INCENP-aurora B kinase-survivin chromosomal passenger complex. EVI-5 is implicated in abnormal states, such as multiple sclerosis and neuroblastoma.

Imunogênio

KLH-conjugated linear peptide corresponding to the human EVI-5.

Aplicação

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

This EVI-5 antibody is validated for use in IHC & WB for the detection of the EVI-5 protein.

Western Blot Analysis: 2 µg/mL from a representative lot detected EVI-5 in NIH/3T3 nuclear extract.

Qualidade

Evaluated by Immunohistochemistry in mouse and rat brain tissues.

Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected EVI-5 in mouse and rat brain tissues.

Descrição-alvo

~95 kDa observed. Uncharacterized bands may be observed at ~31 kDa, ~17 kDa, and ~15 kDa in some cell lysates. This protein is known to have three potential phosphorylation sites.

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Mouse and rat brain tissues

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable