ABF188

Anti-IRF-1

from rabbit, purified by affinity chromatography

• Sinônimo(s): Interferon regulatory factor 1, IRF1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human, rat
• reatividade da espécie (prevista por homologia): mouse (based on 100% sequence homology)
• embalagem: antibody small pack of 25 μg
• técnica(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• nº de adesão NCBI: NP_002189.1
• nº de adesão UniProt: P10914
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... IRF1(3659)

Descrição

Descrição geral

Interferon regulatory factor 1 (UniProt: P10914; also known as IRF-1) is encoded by the IRF1 gene (Gene ID: 3659) in human. IRF-1 is a transcriptional regulator that is expressed constitutively in all cells types except early embryonal cells. It can exist as a monomer and as a homodimer. It displays remarkable functional diversity in the regulation of cellular responses, including the regulation of interferon (IFN) and IFN-inducible genes, host response to viral and bacterial infections. It also regulates many genes expressed during hematopoiesis, inflammation, immune responses, cell proliferation and differentiation, and growth arrest and apoptotic cell death following DNA damage. IRF-1 is reported to stimulate p53/TP53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53/TP53. Its DNA-binging domains are located in the first 115 amino acids of N-terminal region and contains a series of five well-conserved tryptophan-rich repeats. IRF-1 can be phosphorylated by casein kinase 2, which positively regulates its activity. Mutations in IRF1 gene are reported to cause gastric cancer. (Ref.: Dou, L., et al. (2014). Hum. Immunol. 75(11); 1110-1114).

Especificidade

This rabbit polyclonal antibody detects Interferon Regulaotry Factor 1 in human cells. It targets an epitope within 14 amino acids from the N-terminal half.

Imunogênio

KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal half of human Interferon Regulatory Factor 1.

Aplicação

Anti-IRF-1, Cat. No. ABF188, is a rabbit polyclonal lantibody that detects Interferon regulatory factor 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Inflammation & Immunology

Western Blotting Analysis: A 1:500 dilution from a representative lot detected IRF-1 in Jurkat cell lysate.

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected IRF-1 in human stomach cancer and human small intestine tissue sections.

Qualidade

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected IRF-1 in PC12 cell lysate.

Descrição-alvo

~48 kDa observed; 36.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

forma física

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable