ABC457

Anti-dUTPase Antibody

from rabbit

• Sinônimo(s): Deoxyuridine 5′-triphosphate nucleotidohydrolase, mitochondrial, dUTPase, dUTP pyrophosphatase, dUTPase
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: human
• técnica(s): western blot: suitable
• nº de adesão NCBI: NP_001020419
• nº de adesão UniProt: P33316
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... DUT(1854)

Descrição

Descrição geral

dUTPase, also known as dUTP pyrophosphatase, and encoded by the gene DUT, is a critical enzyme involved in nucleotide metabolism for the production of dUMP, the immediate precursor of thymidine nucleotides. dUTPase normally can also function to decrease the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA and cause mutations. dUTPase is localized to the nucleus and mitochondrial locations. dUTPase is expressed in numerous tissues and has multiple isoforms. Some isoforms are constitutively expressed while others match DNA replication cycles. Because dUTPase plays a pivotal role in regulating cellular dUTP pools, this enzyme could have profound effects on the efficacy of agents that target thymidylate biosynthesis and dUTPase expression is being examined as a prognostic marker for overall survival and response to therapy in colon and other cancers where thymidylate therapy is used.

Imunogênio

Recombinant protein corresponding to human dUTPase.

Aplicação

Anti-dUTPase Antibody is a highly specific rabbit polyclonal antibody, that targets dUTPase & has been tested in western blotting.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 2 µg/mL from a representative lot detected dUTPase in 10 µg of A431, HEK293, and HeLa cell lysates and human placenta tissue lysate.

Western Blotting Analysis: A representative lot from an independent laboratory detected dUTPase in MDA-MB231, MCF-7, H184B5F5/M10 cell lysate (Hu, C. M., et al. (2012). Cancer Cell. 22(1):36-50.).

Qualidade

Evaluated by Western Blotting in MDA-MB-231 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected dUTPase in 10 µg of MDA-MB-231 cell lysate.

Descrição-alvo

~18 kDa observed. The expected molecular weight of this protein is 27 kDa However, this protein has been observed at 18 kDa (Prof. Z. F. Chang, National Yang-Ming University). Uncharacterized band(s) may be observed in some cell lysates.

forma física

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable