AB9074

Anti-IP3 Receptor 2 Antibody

serum, Chemicon^®

• Sinônimo(s): Inositol 1,4,5-Trisphosphate Receptor 2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: serum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: human, mouse
• fabricante/nome comercial: Chemicon^®
• técnica(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• nº de adesão NCBI: NM_002223.2
• nº de adesão UniProt: Q14571
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... ITPR2(3709)

Descrição

Especificidade

IP3 Receptor 2.

Imunogênio

Synthetic peptide from the C terminus of human IP3 Receptor 2.

Aplicação

Detect IP3 Receptor 2 using this Anti-IP3 Receptor 2 Antibody validated for use in IC, IH & WB.

Research Category
Signaling

Research Sub Category
Lipid Signaling

Western blot: 1:1,000 using ECL. The antibody reacts with the 250 kDa IP3 Receptor 2 protein in microsomal preparations. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel or 5% PAGE. For microsomal methods and blotting procedure similar antibodies can be found in: Perez, et al. J Biol. Chem. 272:23961

http://www.jbc.org/cgi/reprint/272/38/23961?ijkey=35bcf508a3025fc8bf8203ea3805122eec0caf1e )

Immunocytochemistry: 1:1,000

Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.

Optimal working dilutions must be determined by the end user.

forma física

Rabbit serum. Liquid.

Armazenamento e estabilidade

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Nota de análise

Control
Western blot = adult mouse liver.

IHC = adult mouse hepatocytes (adult mouse cerebellum Purkinje cells will be negative).

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable