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AB3203

Sigma-Aldrich

Anti-Tubulin Antibody, δ 2

Chemicon®, from rabbit

Sinônimo(s):

Anti-α-tubulin, Tubulin antibody

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

affinity purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

sea urchin, echinoderm, plant

não deve reagir com

Saccharomyces cerevisiae

reatividade da espécie (prevista por homologia)

mammals

fabricante/nome comercial

Chemicon®

técnica(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... TUBD1(51174)

Descrição geral

Tubulin, the dimeric subunit of microtubules, is submitted to post translational modifications including cyclic tyrosine removal and addition at the COOH terminus of the alpha subunit. The cycle involves two enzymes, TTL (Tubuline Tyrosine Ligase) and Tubulin carboxypeptidase, and generates two major forms: tyrosinated tubulin and detyrosinated tubulin (Glu-tubulin). Removal of the COOH-terminal Glu residue from the alpha chain of Glu-tubulin generates the delta2-tubulin form.

Especificidade

The antibody specifically recognizes the delta 2 form of the tubulin alpha chain (Paturle-Lafanechere et al. 1991).

SPECIES REACTIVITY:

Mammals, sea urchin, plants, not yeast (Lafanechere et al. 1994).

Imunogênio

Peptide corresponding to the C-terminal of delta 2 tubulin.

Aplicação

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Tubulin Antibody, δ 2 is validated for use in IH(P), WB, IC, IH for the detection of Tubulin.
Western blot: 1:1,000

Immunohistochemistry and EM on either frozen or paraffin embedded, fixed tissue (Mialhe et al. 2001): 1:500

Optimal working dilutions must be determined by the end user.

forma física

Affinity purified immunoglobulin. Liquid in PBS.

Armazenamento e estabilidade

Maintain at 2-8°C in undiluted for up to 6 months from date of receipt.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

Robert O'Hagan et al.
Current biology : CB, 21(20), 1685-1694 (2011-10-11)
Posttranslational modifications (PTMs) such as acetylation, detyrosination, and polyglutamylation have long been considered markers of stable microtubules and have recently been proposed to guide molecular motors to specific subcellular destinations. Microtubules can be deglutamylated by the cytosolic carboxypeptidase CCP1. Loss
Tracy Chong et al.
BMC developmental biology, 11, 69-69 (2011-11-15)
The freshwater planarian Schmidtea mediterranea exhibits two distinct reproductive modes. Individuals of the sexual strain are cross-fertilizing hermaphrodites with reproductive organs that develop post-embryonically. By contrast, individuals of the asexual strain reproduce exclusively by transverse fission and fail to develop
Kinesin-13 and tubulin posttranslational modifications regulate microtubule growth in axon regeneration.
Ghosh-Roy, A; Goncharov, A; Jin, Y; Chisholm, AD
Developmental Cell null
Epigenetic characterization of the vegetative and floral stages of azalea buds: dynamics of DNA methylation and histone H4 acetylation.
Monica Meijon,Luis Valledor,Estrella Santamaria,Pilar S Testillano,Ma Carmen Risue?o et al.
Journal of Plant Physiology null
Isolation rearing induces recognition memory deficits accompanied by cytoskeletal alterations in rat hippocampus.
Bianchi, M, et al.
The European Journal of Neuroscience, 24, 2894-2902 (2006)

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