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Documentos Principais

8.55054

Sigma-Aldrich

EDANS NovaTag®_resin

Novabiochem®

Sinônimo(s):

EDANS-MPB-AM resin

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About This Item

Código UNSPSC:
12352116
NACRES:
NA.22

Nível de qualidade

200

linha de produto

NovaSyn® TG
Novabiochem®

Formulário

beads

adequação da reação

reaction type: Fmoc solid-phase peptide synthesis

fabricante/nome comercial

Novabiochem®

aplicação(ões)

peptide synthesis

grupo funcional

amine

temperatura de armazenamento

15-25°C

Descrição geral

The EDANS/dabcyl fluorophore-quencher pair is one of the most commonly-used for FRET peptides, owing to excellent spectral overlap between the emission spectrum of EDANS and absorbance spectrum of dabcyl [1]. The introduction of the EDANS moiety is traditionally achieved either by coupling of a peptide fragment to EDANS in solution [1, 2] or through the use of a pre-formed derivative, such as Fmoc-Glu(EDANS)-OH [3]. Novabiochem′s EDANS resin enables for the first time the direct synthesis of C-terminally EDANS-labeled peptides by solid phase synthesis [4]. Loading of the first residue requires acylation of the resin-bound secondary amine and is, therefore, best carried out using HATU activation. Chain extension and cleavage can then be effected using standard methods. EDANS NovaTag resin has been recently employed to prepare fluorescently labeled aminoalkane diphenyl phosphonate affinity probes for chymotrypsin- and elastase-like serine proteases [5].

Associated Protocols and Technical Articles
Protocols for Loading of Peptide Synthesis Resins Literature references

[1] G. T. Wang, et al. (1990) Tetrahedron Lett., 31, 6493.
[2] C. Garcia-Echeverria & D. H. Rich (1992) Febs Lett., 297, 100.
[3] J. W. Drijfhout, et al. in ′Peptides, Chemistry, Structure& Biology, Proc. 14th American Peptide Symposium′, P. T. P. Kaumaya &R. S. Hodges (Eds), Kingswinford, Mayflower Scientific Ltd., 1996, pp. 129.
[4] J. Beythien & P. White (2005) Tetrahedron Lett., 46,101.
[5] B. F. Gilmore, et al. (2009) Bioconjugate Chem., 20, 2098.

Ligação

Replaces: 04-12-3904

Nota de análise

Color (visual): white to yellow to brown
Appearance of substance (visual): beads
Loading (determined from the substitution of the Fmoc-Leu loaded resin): 0.30 - 0.60 mmol/g
Swelling Volume (in DMF): lot specific result
The polymer matrix is copoly styrene - 1 & DVB 100 - 200 mesh

Informações legais

NOVASYN is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVATAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Artigos

Peptide-Labeling

The spectral properties of Novabiochem®’s chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry. Novabiochem® range offers one of the most extensive ranges of labeling reagents for the synthesis of such peptides, including the unique NovaTag™ resins for the production of C-terminally-labeled peptide

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