5.34329

H₂S Fluorescent Probe, P3

• Sinônimo(s): H₂S Fluorescent Probe, P3, ( E)-2-(3-(6-(2-Hydroxyethylamino)naphthalen-2-yl)-3-oxoprop-1-enyl)-3,5-dimethoxybenzaldehyde, Hydrogen Sulfide Fluorescent Probe, P3, Probe P3, (E)-2-(3-(6-(2-Hydroxyethylamino)naphthalen-2-yl)-3-oxoprop-1-enyl)-3,5-dimethoxybenzaldehyde, Hydrogen Sulfide Fluorescent Probe, P3, Probe P3
• Fórmula empírica (Notação de Hill): C₂₄H₂₃NO₅
• Peso molecular: 405.44
• Código UNSPSC: 12352202
• NACRES: NA.28

Propriedades

• Ensaio: ≥98% (HPLC)
• Nível de qualidade: 100
• forma: solid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; protect from light
• cor: orange
• solubilidade: DMSO: 100 mg/mL
• fluorescência: λ_ex 375 nm; λ_em 505 nm
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

A cell-permeable, non-toxic, non-fluorescent naphthalenyl-dimethoxybenzaldehyde enone compound that acts as a highly specific, sensitive, fast-responsive H₂S fluoroprobe (signal saturation at ≤ 5 min with a detection limit of 50 nM). Dose-dependently detects hydrogen sulfide levels under physiological conditions and readily fluoresces. Displays little to no reactivity towards GSH, Cys, H₂O₂, citrate and iodide (Hepes pH 7.4, containing 10% FBS). Also shown to be useful as a two-photon probe for imaging endogeneous H₂S levels in HeLa cells. A thiol specific fluorescent probe is also available (Cat. No. 595504).

A cell-permeable, non-toxic, non-fluorescent naphthalenyl-dimethoxybenzaldehyde enone compound that acts as a highly specific, sensitive, fast-responsive H₂S fluoroprobe (signal saturation at ≤ 5 min with a detection limit of 50 nM). Dose-dependently detects hydrogen sulfide levels under physiological conditions and readily fluoresces. Displays little to no reactivity towards GSH, Cys, H₂O₂, citrate and iodide (Hepes pH 7.4, containing 10% FBS). Also shown to be useful as a two-photon probe for imaging endogeneous H₂S levels in HeLa cells. A thiol specific fluorescent probe is also available (Cat. No. 595504).

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Ações bioquímicas/fisiológicas

Cell permeable: yes

Primary Target
hydrogen sulfide

Reversible: no

Embalagem

Packaged under inert gas

Advertência

Toxicity: Regulatory Review (Z)

Reconstituição

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Outras notas

Singha, S., et al. 2015. Anal. Chem.87, 1188.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable