5.09290

G 418 Sulfate Salt

OmniPur^® Grade, >=730 µg/mg

• Sinônimo(s): OmniPur^® G418 Sulfate, Cell Culture Tested, NMD Inhibitor I
• Fórmula empírica (Notação de Hill): C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
• Número CAS: 108321-42-2
• Peso molecular: 692.71
• Número MDL: MFCD00058314
• Código UNSPSC: 12352200

Propriedades

• Nível de qualidade: 200
• Ensaio: ≥90% (HPLC)
• forma: powder
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• cor: white
• traços de cátion: heavy metals: ≤100 ppm
• temperatura de armazenamento: 10-30°C
• InChI: 1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
• chave InChI: UHEPSJJJMTWUCP-NKCAIAFTSA-N

Descrição

Descrição geral

Aminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis, is toxic to bacteria, yeast, protozoans, helminths, higher plant and mammalian cells. Used in molecular genetics as a selective agent for the bacterial neo^r/kan^r genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.

Aminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neo^r/kan^r genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.

Ações bioquímicas/fisiológicas

Assay: ≥730 µg/mg

Advertência

Toxicity: Standard Handling (A)

Reconstituição

Solubilization in H₂O at 100 mg/ml yields a clear, colorless solution.

Outras notas

Ethier, S.P., and Taback, E. 1993. Cancer Lett.74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol.7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol.133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol.163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun.101, 1031.
Jimenez, A., and Davies, J. 1980. Nature287, 869.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

OMNIPUR is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H317 - H334
• Declarações de precaução: P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312
• Classificações de perigo: Resp. Sens. 1 - Skin Sens. 1
• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 3