444234

MMP-9-Lipocalin-TIMP-1 Complex, Human Neutrophil

• Sinônimo(s): Matrix Metalloproteinase 9-Lipocalin-TIMP-1 Complex
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• Ensaio: ≥90% (SDS-PAGE)
• Nível de qualidade: 100
• Formulário: liquid
• atividade específica: ≥600 mU/mg protein
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• atividade externa: other MMP activity, none detected
• Condições de expedição: wet ice
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

Native MMP-9-Lipocalin-TIMP-1 complex from stimulated human neutrophils. The ternary MMP-9/lipocalin/TIMP-1 complex behaves like the Pro-MMP-9/TIMP-1 complex, acting as an inhibitor of active MMPs. Following activation, the complex acts as a gelatinase, with reduced activity.

Native MMP-9-Lipocalin-TIMP-1 complex from stimulated human neutrophils. The ternary MMP-9/lipocalin/TIMP-1 complex behaves like the progelatinase B/TIMP-1 complex in acting as an inhibitor of active MMPs. Following activation, the complex acts as a gelatinase with reduced activity.

Note: 1 mU = 1 milliunit.

Advertência

Toxicity: Standard Handling (A)

Definição da unidade

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.

forma física

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% Brij^® 35 Detergent, 0.05% NaN₃, pH 7.0.

Nota de preparo

Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstituição

Following initial thaw, aliquot and freeze (-70°C).

Outras notas

Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.

Informações legais

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable