42600-M
Ultrafree® Centrifugal Filter, 0.5 mL Sample Volume
Sinônimo(s):
DA Centrifugal Filter Unit, DA Centrifugal Filter Unit For DNA extraction, Ultrafree-DA Centrifugal Filter Unit, 0.5 mL
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
41104916
eCl@ss:
36100101
Produtos recomendados
Materiais
polypropylene
Nível de qualidade
esterilidade
non-sterile
fabricante/nome comercial
Ultrafree®
Parâmetros
0.5 mL sample volume
50 °C max. temp.
técnica(s)
DNA extraction: suitable
Comprimento
45 mm
Diâmetro
10.7 mm
área de filtração
0.2 cm2
Condições de expedição
ambient
Descrição geral
Recover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin. The kit consists of a pre-assembled filter device with an agarose gel nebulizer, a microcentrifuge vial, and modified TAE gel extraction buffer (dry).
The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.
Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product.
Performance
100 78
700 71
1000 77
2027 47
4361 35
9416 32
23130 29
Protocol
1. Excise the band of interest after routine electrophoretic analysis of PCR products or other DNA through an agarose gel.
2. Place the gel slice into the pre-assembled device. Note: Protocol is not compatible with low melting point agarose.
3. Spin at 5,000 x g for 10 minutes to extract gel slurry into filter cup.
4. DNA can be stored in the capped filtrate vial after discarding the sample filter cup and gel nebulizer.
The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.
Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product.
- Minimal hands-on time
- 10-minute spin
- Fully-functional DNA
Performance
Typical DNA Recoveries from Agarose Gels
DNA Size (bp) Percent DNA Recoveries
100 78
700 71
1000 77
2027 47
4361 35
9416 32
23130 29
Protocol
1. Excise the band of interest after routine electrophoretic analysis of PCR products or other DNA through an agarose gel.
2. Place the gel slice into the pre-assembled device. Note: Protocol is not compatible with low melting point agarose.
3. Spin at 5,000 x g for 10 minutes to extract gel slurry into filter cup.
4. DNA can be stored in the capped filtrate vial after discarding the sample filter cup and gel nebulizer.
Aplicação
Fixed-angle microfuge rotor w/maximum 5,000 x g
Informações legais
Ultrafree is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica