344905

FURA 2/AM

Cell-permeable ester form of FURA 2.

• Sinônimo(s): FURA 2/AM, 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2ʹ-amino-5ʹ-methylphenoxy)-ethane-N,N,NʹNʹ-tetraacetic Acid Pentaacetoxymethyl Ester
• Fórmula empírica (Notação de Hill): C₄₄H₄₇N₃O₂₄
• Número CAS: 108964-32-5
• Peso molecular: 1001.85
• Número MDL: MFCD00036976
• Código UNSPSC: 12171500
• NACRES: NA.47

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥95% (HPLC)
• forma: solid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; protect from light
• cor: yellow
• fluorescência: λ_ex 335 nm (high); λ_ex 362 nm (low); λ_em 505 nm (high); λ_em 512 nm (low)
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C
• InChI: 1S/C44H47N3O24/c1-25-7-8-32(46(16-38(53)65-20-60-26(2)48)17-39(54)66-21-61-27(3)49)35(13-25)58-11-12-59-42-31-14-36(43-45-15-37(71-43)44(57)69-24-64-30(6)52)70-34(31)10-9-33(42)47(18-40(55)67-22-62-28(4)50)19-41(56)68-23-63-29(5)51/h7-10,13-15H,11-12,16-24H2,1-6H3
• chave InChI: UTBYGIDPBQOHNT-UHFFFAOYSA-N

Descrição

Descrição geral

Cell permeant ester form of FURA 2 (Cat. No. 344900). The active dye is rapidly released in the cell by cytoplasmic esterases. Fluorescent calcium chelator (K_d = 224 nM) that offers 30 times greater fluorescence intensity than QUIN 2 (Cat. No. 551826). Can be used as a probe in many cellular systems, including cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max.: 362 nm, emission max.: 512 nm) differ from those during high calcium conditions (excitation max.: 335 nm, emission max.: 505 nm). Note: 1 set = 10 x 100 µg.

Cell-permeable ester form of FURA 2. Note: 1 set = 10 x 100 µg.

PLURONIC^® F-127, PROTEIN GRADE Detergent (Cat. No. 540025, available separately) is a mild, low-toxicity detergent that can aid in the solubilization of FURA 2/AM.

Ações bioquímicas/fisiológicas

Cell permeable: yes

Primary Target
Ratiometric calcium indicator

Product does not compete with ATP.

Reversible: no

Embalagem

Packaged under inert gas

Advertência

Toxicity: Standard Handling (A)

Nota de preparo

Further dilute with aqueous buffers just prior to use.

Reconstituição

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 1 month at -20°C.

Outras notas

Davis, M.J., et al. 1992. Am. J. Physiol. 263, H1292.
Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol.47, 226.
Schwartz, Y., et al. 1992. Endocrinology131, 772.
Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.
Janconi, M.E., et al. 1990. J. Cell Biol.110, 1555.
Tsien, R.Y. 1980. Biochemistry19, 2396.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pluronic is a registered trademark of BASF

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable