235400

Caspase-3 Substrate I, Colorimetric

Colorimetric substrate for caspase-3 (Km = 9.7 µM) and related cysteine proteases.

• Sinônimo(s): Caspase-3 Substrate I, Colorimetric, Ac-DEVD- pNA, Ac-DEVD-pNA
• Fórmula empírica (Notação de Hill): C₂₆H₃₄N₆O₁₃
• Número CAS: 1177131-27-9
• Peso molecular: 638.58
• Código UNSPSC: 12352200

Propriedades

• Ensaio: ≥95% (HPLC)
• Formulário: lyophilized
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; desiccated; protect from light
• solubilidade: DMSO: 60 mg/mL
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Colorimetric substrate for CPP32/Apopain/Yama (K_m = 9.7 µM) and related cysteine proteases. Sequence is based on the P1-P4 tetrapeptide cleavage site of poly (ADP-ribose) polymerase (PARP) and includes Asp²¹⁶. CPP32 is the protease responsible for the cleavage of PARP. Can be used with Pro-caspase-3 in in vitro assays to measure caspase-9 activity, since caspase-9 activates pro-caspase-3. Cleavage of the substrate can be monitored at 405 nm (ε = 9160 cm^-1M^-1).

Colorimetric substrate for caspase-3 (K_m = 9.7 µM) and related cysteine proteases. Sequence is based on the P₁-P₄ tetrapeptide cleavage site of poly(ADP-ribose) polymerase (PARP) and includes Asp²¹⁶. Caspase-3 is the protease responsible for the cleavage of PARP. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10. Cleavage of pNA is monitored colorimetrically at ~405 nm.

Ações bioquímicas/fisiológicas

Cell permeable: no

Km = 9.7 µM as colorimetric substrate for caspase-3

Primary Target
Caspas-3

Product does not compete with ATP.

Reversible: no

Advertência

Toxicity: Standard Handling (A)

Sequência

Ac-Asp-Glu-Val-Asp-pNA

Reconstituição

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Outras notas

Cardone, M.H., et al. 1998. Science 282, 1318.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Nicholson, D.W., et al. 1995. Nature376, 37.
Lazebnik, Y.A., et al. 1994. Nature371, 346.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable