204888

Complement C5, Human

Complement C5, Human, CAS 80295-53-0. is a native glycoprotein composed of two non-identical subunits of M.W. 120 kDa (α) and 75 kDa (β) linked by disulfide bonds.

• Sinônimo(s): Complement C5, Human
• Número CAS: 80295-53-0
• Número MDL: MFCD00130840
• Código UNSPSC: 12352202
• NACRES: NA.25

Propriedades

• Nível de qualidade: 200
• Ensaio: ≥95% (SDS-PAGE)
• forma: liquid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• Impurezas: IgG, IgA, IgM, albumin, ceruloplasmin or functionally C3, C6, C7, C8, or C9, trace
• Condições de expedição: wet ice
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

Native, human C5 complement component. Glycoprotein composed of two non-identical subunits of M.W. 120 kDa (α) and 75 kDa (β) linked by disulfide bonds. Present in normal human serum at 70 µg/ml. Activation of complement via either the classical or alternative pathway can result in the assembly of C5-cleaving enzymes (C5 convertases) on target surfaces. Both C5 convertases cleave the C5 α-chain at peptide bond 74 resulting in production of C5a (M.W. 11,200) and C5b fragments (M.W. 185,000). Released C5a peptide is one of the three complement-derived anaphylatoxins. C5b fragment combines with C6, C7, C8, and C9 to form lytic C5b-9 complement membrane attack complex (MAC).

Native, human C5 complement component. Glycoprotein composed of two non-identical subunits of M.W. 120,000 (α) and 75,000 (β) linked by disulfide bonds. Present in normal human serum at 70 µg/ml. Activation of complement via either the classical or alternative pathway can result in assembly of C5-cleaving enzymes (C5 convertases) on target surfaces. Both C5 convertases cleave the C5 α-chain at the peptide bond 74, resulting in production of C5a (M.W. 11,200) and C5b fragments (M.W. 185,000). Released C5a peptide is one of the three complement-derived anaphylatoxins. C5b fragment combines with C6, C7, C8, and C9 to form the lytic C5b-9 complement membrane attack complex (MAC).

Ações bioquímicas/fisiológicas

≥100,000 C₅H₅0 units/mg; ≥60% of the C5 activity in normal human serum on a mg/mg basis

Embalagem

Please refer to vial label for lot-specific concentration.

Advertência

Toxicity: Standard Handling (A)

forma física

In PBS, pH 7.2.

Nota de preparo

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstituição

Following initial thaw, aliquot and freeze (-70°C).

Outras notas

Janatova, J. 1988. Methods Enzymol. 162, 579.
Wetsel, R.A., et al. 1980. J. Immunol. Methods 35, 319.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable