1.25080

Fractogel^® EMD DMAE (M)

MiniChrom Fractogel^® DMAE (M), volume 5 mL, prepacked column

• Sinônimo(s): MiniChrom Column Fractogel^® DMAE (M), 5 ml
• Código UNSPSC: 41115711

Propriedades

• ligand: diethyl-(2-hydroxypropyl)aminoethyl
• Materiais: polypropylene housing
• linha de produto: Fractogel^®
• forma: prepacked column
• Parâmetros: ≤8 bar pressure; ~170 cm/hr max. flow rate
• column I.D. × L: 8 mm × 100 mm
• volume: 5 mL
• Grupo ativo da matriz: Methacrylate
• tamanho médio de partícula: 40-90 μm
• capacidade: ~100 mg, BSA binding capacity
• aplicação(ões): gene therapy; recombinant protein; vaccine development
• técnica de separação: anion exchange
• temperatura de armazenamento: 15-25°C

Descrição

Descrição geral

Weak cation exchange Fractogel^® EMD DMAE (M) resin in a convenient pre-packed 5ml column

Características e benefícios

MiniChrom Column Fractogel^® DMAE (M), 5 ml enables:

• Excellent binding to large viruses and plasmid DNA
• Homogenous binding with high selectivity and purity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the degree of the ionization of the functional groups of the exchanger
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Informações legais

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany