09-258

Anti-phospho-PAK1 (Ser199/Ser204) Antibody

Upstate^®, from rabbit

• Sinônimo(s): Anti-IDDMSSD, Anti-PAKalpha, Anti-alpha-PAK, Anti-p65-PAK
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• reatividade da espécie (prevista por homologia): mouse (100% immunogen homology), rat (100% immunogen homology)
• fabricante/nome comercial: Upstate^®
• técnica(s): western blot: suitable
• nº de adesão NCBI: NP_002567
• nº de adesão UniProt: Q13153
• Condições de expedição: dry ice
• modificação pós-traducional do alvo: phosphorylation (pSer199/pSer204)
• Informações sobre genes: human ... PAK1(5058) ; mouse ... Pak1(18479) ; rat ... Pak1(29431)

Descrição

Descrição geral

p21-activated kinases (PAK) are Ser/Thr kinases, which are activated by, and are effectors of Rho family of GTPases. As such, they are implicated in Actin polymerization and activation of the stress-activated kinase cascades. PAK1 is phosphorylated by cdk5, resulting in its inhibition. PAK2 can be activated by Caspase-cleavage in addition to p21-binding, implicating it in cytoskeletal changes during apoptosis. PAK3 is expressed at high levels in post-mitotic neurons of the developing post-natal cerebral cortex and hippocampus, and is the mutant gene thought responsible for non-syndromic X-linked mental retardation.

Especificidade

Recognizes PAK1 phosphorylated on Ser199 and Ser204 (human)/Ser198 and Ser203 (mouse). Immunogen sequence is higly homolgous (greater than 90%) with PAK2 and PAK3.

Imunogênio

Amino Acids encompassing and including phosphorylated Ser199 and Ser204 of human PAK1. Sequence is highly homologous (greater than 90%) to PAK2 and PAK3.

Epitope: Ser199/Ser204 (Central)

Aplicação

Detect phospho-PAK1 (Ser199/Ser204) using this Anti-phospho-PAK1 (Ser199/Ser204) Antibody validated for use in WB.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Qualidade

Routinely evaluated by western blot on untreated and pervanadate-treated Jurkat cell lysates.

Descrição-alvo

65 kDa

forma física

Affinity purified rabbit polyclonal IgG in storage buffer containing PBS with 0.05% sodium azide in 50% glycerol

Double affinity purification over both non-phospho-peptide column followed by phopshorylated peptide column.

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable