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Merck
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Key Documents

07-425-I

Sigma-Aldrich

Anti-Neurogranin

from rabbit, purified by affinity chromatography

Sinônimo(s):

Ng, RC3

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

rat, human

reatividade da espécie (prevista por homologia)

goat (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology)

embalagem

antibody small pack of 25 μg

técnica(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

Isotipo

IgG

nº de adesão NCBI

nº de adesão UniProt

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... NRGN(4900)

Descrição geral

Neurogranin (UniProt: Q92686; also known as Ng, RC3) is encoded by the NRGN gene (Gene ID: 4900) in human. Neurogranin is abundantly expressed in cerebral cortex, hippocampus, amygdala, and striatum. In neurons, it is found concentrated at dendritic spines. It acts as a third messenger and serves as a substrate for protein kinase C-mediated molecular cascades during synaptic development and remodeling. It contains an IQ domain (aa 26-347) and a collagen-like domain (aa 48-78). The IQ domain mediates its interaction with calmodulin and phosphatidic acid (PA). Neurogranin is intrinsically unstructured, however, upon binding with calmodulin, the IQ domain adopts a helical conformation. Neurogranin is phosphorylated at serine 36 by protein kinase C and following phosphorylation neurogranin is unable to bind either calmodulin or PA, which can lead to decline in memory-associated functions. Higher levels of neurogranin have also been reported in brains of elderly and is considered a factor in memory decline, independent of Alzheimer s disease. (Ref.: Javier Dı´ez-Guerra, F. (2010). Life. 62(8): 597-606; Casaletto, KB et al. (2017). Neurology 89(17); 1782-1788).

Especificidade

This rabbit polyclonal antibody sepecifically detects Neurogranin. It targets an epitope with in 12 amino acids from the C-terminal region.

Imunogênio

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Neurogranin.

Aplicação

Anti-Neurogranin Antibody, Cat. No. 07-425-I, is a highly specific rabbit polyclonal antibody that targets Neurogranin and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Neurogranin in human and rat brain tissue sections.
Research Category
Neuroscience

Qualidade

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Neurogranin in rat brain tissue lysate.

Descrição-alvo

~15 kDa observed; 7.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

forma física

Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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