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07-307

Sigma-Aldrich

Anti-phospho-STAT1 (Tyr701) Antibody

Upstate®, from rabbit

Sinônimo(s):

Transcription factor ISGF-3 components p91/p84, signal transducer and activator of transcription 1, signal transducer and activator of transcription 1, 91kD, signal transducer and activator of transcription 1, 91kDa, signal transducer and activator of tr

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

affinity purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

mouse, chicken, human, rat

fabricante/nome comercial

Upstate®

técnica(s)

western blot: suitable

Isotipo

IgG

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

modificação pós-traducional do alvo

phosphorylation (pTyr701)

Informações sobre genes

human ... STAT1(6772)

Descrição geral

STAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT 1, is activated by a number of different ligands, including IFNalpha, IFNgamma, EGF, PDGF and IL6. Phosphorylation of tyrosine 701 is required for STAT 1 dissociation from IFNGR1, homodimerization, and nuclear translocation. Tyrosine 701 phosphorylation impairment results in loss of STAT 1 functions.

Especificidade

Other species cross-reactivity is unknown.
Phosphorylated STAT1

Imunogênio

Epitope: Tyr701
KLH-conjugated synthetic peptide (C-DDPKRTG[pY]IKTE) corresponding to amino acids 694-705 of mouse STAT1, containing a N-terminal cysteine.

Aplicação

Anti-phospho-STAT1 (Tyr701) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-STAT1 (Tyr701) also known as Transcription factor ISGF-3 components p91/p84 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Qualidade

Routinely evaluated by immunoblot in RIPA lysates from CNTF-treated SK-N-MC cells.

Western Blot Analysis:
A 1:500-1:2000 dilution of this lot detected phosphorylated STAT1 in RIPA lysates from CNTF-treated SK-N-MC cells.

Descrição-alvo

92 kDa

forma física

Immunoaffinity Chromatography
Immunoaffinity purified IgG in 0.02 M PBS containing 0.1% Sodium Azide + 50% Glycerol and 5 mg/mL BSA.

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Nota de análise

Control
Mouse 3T3-L1 adipocytes stimulated with leukemia inhibitory factor (LIF).

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Ana Maria Low-Calle et al.
Virus research, 180, 49-58 (2014-01-01)
Herpes simplex virus type 1 (HSV-1) causes a lytic infection in epithelial cells before being captured and moved via retrograde axonal transport to the nuclei of the sensory neurons of the trigeminal ganglion or dorsal root, where it establishes a
Induction of type I interferons by a novel porcine reproductive and respiratory syndrome virus isolate.
Nan, Yuchen, et al.
Virology, 432, 261-270 (2012)
STAT2 deficiency and susceptibility to viral illness in humans.
Hambleton, S; Goodbourn, S; Young, DF; Dickinson, P; Mohamad, SM; Valappil, M; McGovern et al.
Proceedings of the National Academy of Sciences of the USA null
Precision-cut slice cultures of tumors from MMTV-neu mice for the study of the ex vivo response to cytokines and cytotoxic drugs.
Nirmala Parajuli, Wolfgang Doppler
In Vitro Cellular & Developmental Biology. Animal null
Hepatitis C virus core protein differently regulates the JAK-STAT signaling pathway under interleukin-6 and interferon-gamma stimuli.
Hosui, Atsushi, et al.
The Journal of Biological Chemistry, 278, 28562-28571 (2003)

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