06-821-I

Anti-phospho-PKCepsilon Antibody (Ser729)

from rabbit, purified by affinity chromatography

• Sinônimo(s): PKCepsilon, PKCe, Protein kinase C epsilon type, nPKC-epsilon
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: rat, human, mouse
• técnica(s): western blot: suitable
• nº de adesão NCBI: NP_005391
• nº de adesão UniProt: Q02156
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: phosphorylation (pSer729)
• Informações sobre genes: human ... PRKCE(5581)

Descrição

Descrição geral

Protein Kinase C epsilon (PKC epsilon) is an ~87 kDa (migrates predominantly as 95 kDa in quiescient cells) member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, metastasis and transcriptional regulation. PKC epsilon is an actin cytoskeleton regulating protein whose overexpression confers an oncogenic phenotype by affecting the ras signaling cascade. PKC epsilon is phosphorylated by PDK1 on threonine 566 and is then autophosphorylated on the turn motif threonine 710 and the hydrophobic motif serine 729. Catalytically inactive PKC epsilon is not phosphorylated on serine 729. Phosphorylation of serine 729 is mediated by mTOR-dependent pathways, plays an important role in regulating PKC epsilon subcellular localization, and is responsible for the appearance of the 92 kDa PKC epsilon species.

Especificidade

This antibody demonstrates cross-reactivity against the PKCbeta (Ser661) isoform, PKCdelta (Ser655) isoform, and possibly PKCalpha (Ser729), all of which contain the GF(pS)Y motif.

Imunogênio

Epitope: Phosphorylated Ser729

KLH-conjugated linear peptide corresponding to human PKC phosphorylated at Ser729.

Aplicação

Anti-phospho-PKCepsilon Antibody (Ser729) is a highly specific rabbit polyclonal antibody, that targets PKC epsilon & has been tested in western blotting.

Western Blotting/Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot detected phospho PKCepsilon (Ser729) in 10 µg of NIH/3T3 cell lysate.

Qualidade

Evaluated by Western Blotting/Peptide Inhibition Analysis in C6 cell lysate.

Western Blotting/Peptide Inhibition Analysis: A 1:1,000 dilution of this lot detected phospho PKCepsilon (Ser729) in 10 µg of C6 cell lysate.

Descrição-alvo

~95 kDa and ~80 kDa observed. This protein has been observed at ~95 kDa and ~80 kDa (England, K. and Rumsby, M. G., et al. (2000). Biochem J. 352(Pt 1):19-26.).

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable