05-777-AF647

Anti-Phosphotyrosine Antibody, recombinant clone 4G10^® Antibody, Alexa Fluor^™ 647

clone 4G10, from mouse, ALEXA FLUOR^™ 647

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• conjugado: ALEXA FLUOR^™ 647
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 4G10, monoclonal
• reatividade da espécie (prevista por homologia): all
• técnica(s): immunocytochemistry: suitable
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: phosphorylation (pTyr)

Descrição

Descrição geral

Phosphorylation plays an important role in regulating protein activities and various cellular signaling events. Posttranslational phosphorylation can occur on histidine (pHis), serine (pSer), threonine (pThr), and tyrosine (pTyr) residues. Due to the early advent of anti-pTyr antibodies, tyrosine phosphorylation remains the mostly studied signaling events in all biological research fields. Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Before the availability of anti-pTyr antibodies, protein tyrosine phosphorylation was routiniely detected by time-consuming radioactive labeling. Although radioactive labeling is still considered as more sensitive, advancement in detection technology has greatly improved the detection limits of antibody-based methods. Anti-pTyr antibodies are commonly used in ELISA, immunoprecipitation, immunoblotting (including dot blot and Western blot), as well as indirect immunofluourescent detection of tyrosine phosphorylation in tissue and cell samples.

Especificidade

Recognizes tyrosine-phosphorylated proteins from all species.

Target modification is not species-specific

Imunogênio

Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Purified via heavy chain C-terminus hexa-histidine tag by Nickel affinity matrices prior to conjugation with Alexa Fluor^™ 647.

Aplicação

Anti-Phosphotyrosine Antibody, clone 4G10, Alexa Fluor^™ 647 is a highly validated recombinant mouse monoclonal antibody that targets protein tyrosine phosphorylation and has been tested in Immunocytochemistry.

Research Category
Signaling

This recombinant monoclonal antibody is also available as HRP conjugate (Cat. No. 16-184), agarose conjugate (Cat. No. 16-199), biotin conjugate (Cat. No. 16-204), FITC conjugate (Cat. No. 16-205), PE conjugate (Cat. No. FCMAB323PE), as well as as unconjugated antibody (Cat. No. 05-777) for immunoaffinity purification, flow cytometry, immunocytochemistry, immunoprecipitation, and Western blotting applications.

Qualidade

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected EGF-induced tyrosine phosphorylation of cellular proteins in A431 cells.

Descrição-alvo

Variable depending on the size(s) of the tyrosine-phosphorylated protein(s).

forma física

Protein G purified.

Purified mouse monoclonal IgG2a antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Informações legais

4G10 is a registered trademark of Upstate Group, Inc.

ALEXA FLUOR is a trademark of Life Technologies

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable