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Merck
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Documentos Principais

05-363

Sigma-Aldrich

Anti-Cyclin E Antibody, clone HE12

clone HE12, Upstate®, from mouse

Sinônimo(s):

Anti-CCNE, Anti-pCCNE1

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

100

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

HE12, monoclonal

reatividade de espécies

human

embalagem

antibody small pack of 25 μg

fabricante/nome comercial

Upstate®

técnica(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG

nº de adesão NCBI

NM_001238
NM_057182

nº de adesão UniProt

P24864

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... CCNE1(898)

Descrição geral

Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Especificidade

human p52cyclin E

Imunogênio

Recombinant human cyclin E

Aplicação

Anti-Cyclin E Antibody, clone HE12 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin E & has been validated in IP, WB, ICC.

Qualidade

routinely evaluated in immunoblot on human A431 lysate

Descrição-alvo

52 kDa

Ligação

Replaces: 04-223

forma física

Format: Purified

Armazenamento e estabilidade

2 years at -20°C or 6 months at 4°C

Nota de análise

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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S M Keenan et al.
The Journal of biological chemistry, 276(25), 22404-22409 (2001-04-17)
Activation of cyclin-dependent kinase 2 (CDK2)-cyclin E in the late G(1) phase of the cell cycle is important for transit into S phase. In Chinese hamster embryonic fibroblasts (IIC9) phosphatidylinositol 3-kinase and ERK regulate alpha-thrombin-induced G(1) transit by their effects
Activation of p53 by nutlin leads to rapid differentiation of human embryonic stem cells.
Maimets, T; Neganova, I; Armstrong, L; Lako, M
Oncogene null
Hae-June Lee et al.
Radiation research, 170(5), 579-590 (2008-10-31)
The aim of this work was to identify specific genes involved in rat mammary tumors induced by dimethylbenz(a)anthracene (DMBA) or radiation. More TUNEL- and PCNA-positive cells were present in mammary tumors induced by radiation than in tumors induced by DMBA
Inês Gomes et al.
Oncotarget, 11(19), 1714-1728 (2020-06-02)
The role of RANKL-RANK pathway in progesterone-driven mammary carcinogenesis and triple negative breast cancer tumorigenesis has been well characterized. However, and despite evidences of the existence of RANK-positive hormone receptor (HR)-positive breast tumors, the implication of RANK expression in HR-positive
P Saha et al.
Molecular and cellular biology, 17(8), 4338-4345 (1997-08-01)
Cdc25A, a phosphatase essential for G1-S transition, associates with, dephosphorylates, and activates the cell cycle kinase cyclin E-cdk2. p21CIP1 and p27 are cyclin-dependent kinase (cdk) inhibitors induced by growth-suppressive signals such as p53 and transforming growth factor beta (TGF-beta). We
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