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84655

Sigma-Aldrich

Schiff′s reagent for aldehydes

for microscopy

Synonym(s):

Fuchsin sulfurous acid

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About This Item

UNSPSC Code:
12352300
NACRES:
NA.21

grade

for microscopy

Quality Level

form

liquid

application(s)

food and beverages

General description

Schiff′s reagent is used for the qualitative or quantitative assay of carbohydrates. Periodic acid-Schiff′s reagent (PAS) stain is broadly applicable in histology for visualizing glycogen, glycoproteins, mucins, and other polysaccharides in tissue and cells.

Application

Schiff′s reagent for aldehydes has been used:
  • in microtiter PAS assay to quantify the amount of secreted mucin in Vibrio cholera infected water
  • to stain the colonies of Clostridium perfringens in PGY agar plates to identify plasmalogen-deficient mutants
  • in periodic acid-Schiff′s reagent assay to quantify carbohydrates in a microtiter plate format

Pictograms

Health hazardCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 1B - Met. Corr. 1

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Microtiter plate colorimetric assays are widely used for analysis of carbohydrates and glycoconjugates. However, mucins are often not easily detected, as they have low neutral sugar content. We have adapted and optimised the periodic acid-Schiff's reagent (PAS) staining for microtiter
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