857343

Basic Fuchsin

certified by the Biological Stain Commission, Dye content ≥88 %

• Synonym(s): Basic Parafuchsin, Basic Red 9, Magenta^™ O, Parafuchsin hydrochloride, Paramagenta hydrochloride, Pararosaniline chloride, Pararosaniline hydrochloride
• Empirical Formula (Hill Notation): C₁₉H₁₇N₃ · HCl
• CAS Number: 569-61-9
• Molecular Weight: 323.82
• Colour Index Number: 42500
• Beilstein: 4164603
• EC Number: 209-321-2
• MDL number: MFCD00001657
• UNSPSC Code: 12171500
• PubChem Substance ID: 24888442
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• composition: Dye content, ≥88%
• technique(s): microbe id | staining: suitable
• color: green to dark green
• pH: 1.0-3.1, purple to red
• mp: 268-270 °C (dec.) (lit.)
• λmax: 544 nm
• ε (extinction coefficient): ≥11000 at 235-239 nm in 50% ethanol at 0.003 g/L; ≥17000 at 287-291 nm in 50% ethanol at 0.003 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].Nc1ccc(cc1)C(\c2ccc(N)cc2)=C3/C=CC(=[NH2+])C=C3
• InChI: 1S/C19H17N3.ClH/c20-16-7-1-13(2-8-16)19(14-3-9-17(21)10-4-14)15-5-11-18(22)12-6-15;/h1-12,20H,21-22H2;1H
• InChI key: JUQPZRLQQYSMEQ-UHFFFAOYSA-N

Description

Application

Basic fuchsin has been used as a lignin-detecting fluorogenic dye for fluorescence-based imaging of lignin in the basal internodes of three Brachypodium distachyon ecotypes.

Biochem/physiol Actions

Basic fuchsin belongs to the triphenylmethane group. Combining basic fuchsin with sulfurous acid results in the formation of fuchsin sulfurous acid, also referred to as Feulgen reagent or Schiff′s reagent. This reagent is responsible for the staining of chromosomal DNA. Basic fuchsin is also used for the staining of lignin in plant tissue samples.

Suitability

Certified for use for acid-fast staining with carbol-fuchsin, as ENDO medium, and as Schiff′s reagent in the Periodic Acid Schiff (PAS) and Feulgen techniques.

Legal Information

Magenta is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H350
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 1B
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves