796-0657

Protéines, mélange

application for HPLC

Propriétés

• Description: Separated on Discovery^® BIO PolyMA-WAX
• Technique(s): HPLC: suitable
• Paramètres du test: column: Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U); mobile phase: [A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear); flow rate: 0.5 mL/min; detector: UV, 280 nm; injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
• Adéquation: application for HPLC
• Application(s): life science and biopharma

Description

Remarque sur l'analyse

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.

Informations légales

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Trypsin Inhibitor