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Merck
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Key Documents

SRP2097

Sigma-Aldrich

HIF-1 α, C-terminal activation domain (776-826 human

recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

Synonyme(s) :

HIF-1alpha, HIF1, HIF1-alpha, MOP1, PASD8, bHLHe78

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.26

Source biologique

human

Produit recombinant

expressed in E. coli

Pureté

≥85% (SDS-PAGE)

Forme

frozen liquid

Poids mol.

~6.7 kDa

Conditionnement

pkg of 10 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Concentration

700 μg/mL

Couleur

colorless to clear

Numéro d'accès NCBI

NM_001530

Numéro d'accès UniProt

Q16665

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... HIF1A(3091)

Description générale

The gene encoding it is localized on human chromosome 14q23.2.

Actions biochimiques/physiologiques

During hypoxia, the two subunits of this factor undergo post-translational modifications which in turn promote transactivation.
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. HIF-1α contains two transactivation domains located between amino acids 531 and 826. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. Specific disruption of the HIF-1 pathway is important for exploring its role in tumor biology and developing more efficient weapons to treat cancer. HIF-1alpha is a master regulator of the hypoxic response, and its proangiogenic activities include, but are not limited to, regulation of vascular endothelial growth factor (VEGF). HIF-1alpha protein expression often seen in invasive breast cancer. Recent data demonstrates that HIF-1alpha knockdown reduces tumorigenicity of MCF-7 cells and suggest a promising combination of both anti-HIF-1 strategy and traditional chemotherapy to improve cancer treatment.

Forme physique

Clear and colorless frozen liquid solution

Notes préparatoires

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Multiple Gastrointestinal Stromal and Other Tumors Caused by Platelet-Derived Growth Factor Receptor a Gene Mutations: A Case Associated with a Germline V561D Defect
Barbara Pasini
The Journal of Clinical Endocrinology and Metabolism, 92(9) (2007)
Regulation of Hypoxia-Inducible Factor 1a Expression and Function by the Mammalian Target of Rapamycin
Christine C. Hudson
Molecular and Cellular Biology, 22(20), 7004-7014 (2002)
Knockdown of hypoxia-inducible factor-1alpha in breast carcinoma MCF-7 cells results in reduced tumor growth and increased sensitivity to methotrexate.
Li J
Biochemical and Biophysical Research Communications, 342(4), 1341-1351 (2006)
Hypoxia-Inducible Factor (HIF)-1 Regulatory Pathway and its Potential for Therapeutic Intervention in Malignancy and Ischemia
Jennifer E. Ziello
The Yale Journal of Biology and Medicine, 80(2), 51-60 (2007)
Vascular endothelial growth factor (VEGF) regulation by hypoxia inducible factor-1 alpha (HIF1A) starts and peaks during endometrial breakdown, not repair, in a mouse menstrual-like model
Xihua Chen
Human Reproduction, 30(9), 2160-2170 (2015)
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