SRP2054

PPARα, ligand binding domain (170-468) human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Synonyme(s) : MGC2237, MGC2452, NR1C1, PPAR, PPARalpha, hPPAR
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.26

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Étiquette/Marqueur: His tagged (N-terminal)
• Pureté: ≥80% (SDS-PAGE)
• Forme: frozen liquid
• Poids mol.: ~39.4 kDa
• Conditionnement: pkg of 10 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Concentration: 850 μg/mL
• Couleur: clear colorless
• Numéro d'accès NCBI: NM_005036 Y
• Numéro d'accès UniProt: Q07869
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... PPARA(5465)

Description

Actions biochimiques/physiologiques

There is evidence that a group of closely related nuclear receptors, called peroxisome proliferator-activated receptors (PPARs), may be involved in chronic diseases such as diabetes, obesity, artherosclerosis and cancer. The PPARs were first cloned as the nuclear receptors that mediate the effects of synthetic compounds called peroxisome proliferators on gene transcription. It soon became clear that eicosanoids and fatty acids can also regulate gene transcription through PPARs. They bind a specific element in the promoter region of target genes only as a heterodimer with the receptor for 9- cis retinoic acid, RXR (retinoid X receptor). Binding of the ligand of either receptor can activate the complex, but binding of both ligands simultaneously is more potent. Three PPAR isotypes have been identified: α, β (δ) (also called NUC1) and γ. PPARα is expressed most in brown adipose tissue and liver, then kidney, heart and skeletal muscle. PPARγ is mainly expressed in adipose tissue, and to a lesser extent in colon, the immune system and the retina. PPARβ is found in many tissues but the highest expression is in the gut, kidney and heart. The target genes of PPARα are a relatively homogenous group of genes that participate in aspects of lipid catabolism such as fatty acid uptake through membranes, fatty acid binding in cells, fatty acid oxidation (in microsomes, peroxisomes and mitochondria) and lipoprotein assembly and transport.

Forme physique

Clear and colorless frozen liquid solution

Notes préparatoires

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable