SAB4200814
Anti-TIMP2 antibody, Mouse monoclonal
clone TM8, purified from hybridoma cell culture
Synonyme(s) :
CSC-21K, Metalloproteinase inhibitor 2, Tissue inhibitor of metalloproteinases 2
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Forme d'anticorps
purified from hybridoma cell culture
Type de produit anticorps
primary antibodies
Clone
TM8, monoclonal
Espèces réactives
human
Conditionnement
antibody small pack of 25 μL
Validation améliorée
recombinant expression
Learn more about Antibody Enhanced Validation
Concentration
~1 mg/mL
Technique(s)
immunoblotting: 0.5-1 μg/mL using HEK-293 over expressing human TIMP2 cells extract.
immunofluorescence: 2.5-5 μg/mL using human HeLa cells.
Isotype
IgG2a
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... TIMP2(7077)
Description générale
TIMP2- Tissue Inhibitor of Metalloproteinases 2, also known as CSC-21K, is a member of the TIMP family of matrix metalloproteinases (MMPs) natural inhibitors. These proteins are involved in regulation of activated MMPs and in extracellular matrix degradation. the TIMPs family consists of 4 members which act in a non-selective manner.
Spécificité
Monoclonal Anti-TIMP2 specifically recognizes human TIMP2.
Immunogène
Recombinant TIMP2 protein.
Application
The antibody is recommended to use in various immunological techniques, including Immunoblot (~24 kDa) and immunofluorescence. Detection of the TIMP2 band by Immunoblotting is specifically inhibited by the immunogen.
Actions biochimiques/physiologiques
"The MMPs activity inhibition is mediated directly by blocking the MMPs catalytic zinc ion with the TIMPs N-terminal cysteine residue.1-2 TIMP2 is also highly studied for it MMP-independent effects including cell cycle regulation 2-4 and its involvement in several cancers progression and invasiveness (e.g. renal cell carcinoma3, non-small cell lung cancer (NSCLC)5 and Gastric Cancer6).Due to the pronounced inhibitory activity, the TIMPs are considered as a potential target in designing new inhibitor variants with altered specificity for MMPs and other zinc-dependent extracellular matrix (ECM) proteins.1-2"
Forme physique
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Stockage et stabilité
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Clause de non-responsabilité
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Wei Wang et al.
Scientific reports, 8(1), 9629-9629 (2018-06-27)
To explore the prognostic related factors and mechanisms of gastric cancer (GC), we performed the systematic analysis with integrated bioinformatics tools based on multiple on-line datasets. With uni-variate COX analysis, we screened out 37 survival hazardous genes in GC. Further
Maxim Levin et al.
Biochimica et biophysica acta. Molecular cell research, 1864(11 Pt A), 1927-1939 (2017-06-22)
Enzymatic proteolysis of cell surface proteins and extracellular matrix (ECM) is critical for tissue homeostasis and cell signaling. These proteolytic activities are mediated predominantly by a family of proteases termed matrix metalloproteinases (MMPs). The growing evidence in recent years that
L-J Su et al.
British journal of anaesthesia, 121(2), 350-357 (2018-07-24)
A biomarker test based on a combination of urine tissue inhibitor of metalloproteinases 2 (TIMP-2) and insulin-like growth factor binding protein 7 (IGFBP7) has been used as a potential biomarker of acute kidney injury (AKI). This meta-analysis aimed to evaluate
J-Z Yang et al.
European review for medical and pharmacological sciences, 22(13), 4156-4165 (2018-07-20)
MicroRNAs (miRNAs) have been identified to play a crucial regulatory role in the development and progression of malignant tumors, including lung cancer. However, the function of miR-550a-3p on the progression of non-small cell lung cancer (NSCLC) remains poorly understood. Quantitative
Anitha Vijayan et al.
American journal of kidney diseases : the official journal of the National Kidney Foundation, 68(1), 19-28 (2016-03-08)
Acute kidney injury (AKI) is a serious complication, commonly occurring in the critically ill population, with devastating short- and long-term consequences. Despite standardization of the definition and staging of AKI, early recognition remains challenging given that serum creatinine level is
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