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Key Documents

SAB1100050

Sigma-Aldrich

Anti-LDHA (216-228) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Anti-LDH-M, Anti-LDH1, Anti-Lactate dehydrogenase A, Anti-PIG19

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~37 kDa

Espèces réactives

human

Technique(s)

western blot: 1:500-1:2,000

Numéro d'accès UniProt

P00338

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... LDHA(3939)

Description générale

Lactate dehydrogenase A (LDHA) or the M subunit is a cytosolic enzyme and is abundantly present in the skeletal muscle. Lactate dehydrogenase B (LDHB) or the H subunit is mainly seen in the heart. Both genes are capable of forming homotetramers or heterotetramers. LDHA gene is located on human chromosome 11p15.4.

Immunogène

synthetic peptide corresponding to amino acids 216-228 of human LDHA

Application

Anti-LDHA (216-228) antibody produced in rabbit has been used in:
  • in situ

  • proximity ligation assay (PLA)
  • immuno-staining
  • western blotting

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Actions biochimiques/physiologiques

Lactate dehydrogenase A (LDHA) participates in anaerobic and aerobic glycolysis. It can regenerate nicotinamide adenine dinucleotide (NAD+) from NADH to maintain the ATP synthesis in the absence of adequate oxygen. The N-terminus of LDHA is essential for the stability of the structure.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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