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S5897

Sigma-Aldrich

Sephadex® G-50

BioReagent, for molecular biology, DNA grade, fine

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About This Item

Numéro CAS:
9004-54-0
Numéro MDL:
MFCD00132771
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.52

Qualité

DNA grade
for molecular biology

Niveau de qualité

200

Gamme de produits

BioReagent

Forme

beads

Technique(s)

DNA purification: suitable

Matrice

cross-linked dextran

Gonflement

1 g swells to 9-11 mL

Diamètre des billes

20-80 μm

Dimension de pores

exclusion limit (20 bp dsDNA (rA)20)

pH

2-10

Activité étrangère

DNase, RNase, none detected

Température de stockage

room temp

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

Clé InChI

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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Description générale

Sephadex® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances.

S5897-100G′s updated product number is GE17-0573-02

Application

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality.

Caractéristiques et avantages

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Informations légales

Sephadex is a registered trademark of Cytiva

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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