QR0200

Quantitative RT-PCR ReadyMix^™

One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

• Synonyme(s) : 1-step RT-qPCR mix, Quantitative real-time PCR master mix
• Code UNSPSC : 41106300
• Nomenclature NACRES : NA.55

Propriétés

• Niveau de qualité: 300
• Utilisation: sufficient for 250 reactions
• Caractéristiques: dNTPs included; hotstart
• Technique(s): RT-qPCR: suitable
• Couleur: colorless
• Entrée: purified RNA
• Compatibilité: ABI 5700; ABI 7000; ABI 7300; ABI 7500 Fast; ABI 7500; ABI 7700; ABI 7900 HT ; ABI 7900 HT Fast; ABI 7900; ABI StepOne; ABI StepOnePlus; ABI ViiA 7; Bio-Rad CFX384; Bio-Rad CFX96; Bio-Rad MJ Chromo4; Bio-Rad MJ Opticon 2; Bio-Rad MJ Opticon; Bio-Rad MiniOpticon; Bio-Rad MyiQ; Bio-Rad iCycler iQ; Bio-Rad iQ5; Cepheid SmartCycler; Eppendorf^® Mastercycler ep realplex2 s; Eppendorf^® Mastercycler ep realplex; Illumina Eco qPCR; Qiagen Corbett Rotor-Gene 3000; Qiagen Corbett Rotor-Gene 6000; Qiagen Corbett Rotor-Gene Q; Roche LightCycler 480; Strategene Mx3000P; Strategene Mx3005P; Strategene Mx4000
• Méthode de détection: probe-based
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Quantitative RT-PCR ReadyMix^™ combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart^™ Taq DNA polymerase in a one-step RT-PCR kit designed for the measurement of gene expression. This convenient 2X master mix includes M-MLV RT, JumpStart^™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart^™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. This kit has been formulated for fluorogenic hybridization probe-based detection methods.

Application

Quantitative RT-PCR ReadyMix^™ has been used in the quantification of messenger RNA (mRNA) levels of specific expressed genes by quantitative reverse-transcription polymerase chain reaction (RT-qPCR).

Caractéristiques et avantages

• The master mix allows consistency and reproducibility from one reaction to the next
• Reduced risk of contamination from multiple pipetting steps
• Reduced set-up time as compared to manual or wax Hot Start methods
• JumpStart^™Taq Polymerase reduces primer-dimer and non-specific product formation
• Broad instrument compatibility
• Includes a separate ROX reference dye vial for reaction normalization

Conditionnement

1 kit sufficient for 250 reactions at 20 μL each or for 100 reactions at 50 μL each.

Informations légales

This product is for research use only.

Eppendorf is a registered trademark of Eppendorf AG

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Composants de kit seuls

• Probe Based qRT-PCR ReadyMix 2 X
• Moloney Murine Leukemia Viral Reverse Transcriptase (M-MLV RT) 5000 U

Composants de kit également disponibles séparément

• P2192 10X PCR Buffer, Optimized for routine PCR with MgCl₂ included 1.5 mL/vial
• M8787 Magnesium chloride solution, PCR Reagent, 25 mM MgCI₂ solution for PCR 25 mM
• P2192 10X PCR Buffer, Optimized for routine PCR with MgCl₂ included 100 X

Informations sur la sécurité

• Pictogrammes: GHS07,GHS09
• Mention d'avertissement: Warning
• Mentions de danger: H315,H317,H319,H410
• Conseils de prudence: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Code de la classe de stockage: 12 - Non Combustible Liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable