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MRN70

Sigma-Aldrich

GenElute™ mRNA Miniprep Kit

sufficient for 70 purifications

Synonyme(s) :

GenElute™ mRNA Kit, Gen Elute

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About This Item

Code UNSPSC :
41105501
Nomenclature NACRES :
NA.52

Utilisation

sufficient for 70 purifications

Niveau de qualité

200

Technique(s)

RNA purification: suitable

Température de stockage

15-25°C

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Description générale

Procedures such as cDNA synthesis, expression profiling and others require separation of mRNA from the vastly more abundant rRNA and tRNA. The GenElute mRNA kits provide convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues. For direct mRNA preparation, cells or tissues are disrupted with SDS/proteinase K digestion to release RNA and eliminate RNases. Both kit types use oligo dT30 covalently linked to 1 μm polystyrene beads to capture polyadenylated mRNA by hybridization. The polystyrene beads remain suspended during hybridization, eliminating the need for mixing or rocking, as is common for cellulose or magnetic particles. Polystyrene was also chosen because oligo(dT) polystyrene beads yield cleaner mRNA with fewer stringent washing steps than does the more commonly used oligo(dT) cellulose (2 or 3 wash steps versus 10 or more). With the GenElute kits, mRNA-bead complexes are washed on a microcentrifuge spin filter, and eluted into 10 mM Tris-HCL, pH 7.5. mRNA prepared with either kit is suitable for a variety of downstream applications such as Northern blotting, expression array or chip hybridizations and cDNA synthesis and library construction.

Application

GenElute™ mRNA Miniprep Kit has been used to purify RNA from total RNA and to isolate RNA.
The purified mRNA is ready for Northern analysis, reverse transcription and PCR, labeling for arrays, and other common applications.

Caractéristiques et avantages

  • Quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein
  • Performs well with large or small amounts of tissue or cells and many samples can be simultaneously extracted
  • One of the most effective methods for isolating total RNA. Purifications can be completed in only one hour starting with fresh tissue or cells

Autres remarques

For additional information, please see www.sigma-aldrich.com/mrna.

Informations légales

GenElute is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

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Adrenal gland-dependent augmentation of plasminogen activator inhibitor-1 expression in streptozotocin-induced diabetic mice.
Oishi K
Journal of Thrombosis and Haemostasis, 4(7), 1566-1574 (2006)
Kumaravel Marimuthu et al.
Physiologia plantarum, 167(3), 282-301 (2019-03-19)
Somatic embryogenesis (SE) is a complex stress related process regulated by numerous biological factors. SE is mainly applicable to mass propagation and genetic improvement of plants through gene transfer technology and induced mutations. In banana, SE is highly genome dependent
Transcriptome-wide mapping of N(6)-methyladenosine by m(6)A-seq based on immunocapturing and massively parallel sequencing.
Dominissini D
Nature Protocols, 8(1), 176-189 (2013)
The nuclear retention signal of HPV16 L2 protein is essential for incoming viral genome to transverse the trans-Golgi network.
DiGiuseppe S, Bienkowska-Haba M, Hilbig L, et al.
Virology, 458-459, 93-105 (2014)
Chenjing Zhang et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(1), 122-132 (2020-01-10)
N6-methyladenosine (m6A) modification in RNA has been implicated in diverse biological processes. However, very little is currently known about its role in nociceptive modulation. Here, we found that the level of spinal m6A modification was significantly increased in a mouse
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Articles

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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