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PURE1A

Sigma-Aldrich

Protein A Antibody Purification Kit

sufficient for 10 purifications

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.32

Utilisation

sufficient for 10 purifications

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

The Protein A Antibody Purification Kit provides all of the reagents necessary to isolate monoclonal and polyclonal antibodies.

Spécificité

Capacity/Run (by Species)
Human IgG 20-25 mg
Mouse IgG1 8-12 mg
Mouse IgG2a 10-15 mg
Mouse IgG2b 6-10 mg
Mouse IgG3 15-20 mg
Rabbit IgG 8-12 mg
Goat IgG 2-4 mg
Bovine IgG 8-12 mg

Application

PURE-1A offers Protein A technology in a prepackaged, easy to use kit form. With this kit, milligram quantities of IgG can be purified from serum, ascites, or cell culture supernatants.
Purification of IgG from other species may be possible, however, the researcher will have to determine the suitability of the kit for their application.
The Protein A Antibody Purification Kit is suitable for use with IgGs from human, mouse, rabbit, goat, and bovine species.

Caractéristiques et avantages

  • High capacity - purify up to 8 mg of mouse IgG or 25 mg of human IgG per column run
  • Specific - will only bind IgG
  • Easy to use - antibody is eluted and desalted in a single step, ready to use
  • Gentle - avoids prolonged exposure of the antibody to low pH

Principe

Using our cartridge system, antibodies elute as highly purified proteins at physiological pH. Protein A is a powerful tool for isolation of antibodies from mammalian hosts. Protein A exhibits a high degree of specificity for IgG and ensures an antibody preparation virtually free of IgA, IgM and non-immunoglobulin serum proteins such as albumin.

Composants de kit seuls

Réf. du produit
Description
  • Binding Buffer 225 mL
  • Desalting Cartridge 1 ea
  • Elution Buffer 75 mL
  • HEPES Buffer 225 mL
  • 5 ml Syringe 1
  • 10 ml Syringe 1
  • Protein A Cartridge 1 mL
  • Regeneration Buffer 75 mL
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Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H302,H370

Classification des risques

Acute Tox. 4 Oral - STOT SE 1

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Protocoles

Antibody Purification using Protein A, Protein G, or Protein L Agarose

Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum or ascites the final preparation will contain endogenous host IgG as well as specific antibodies. In general, the presence of this endogenous IgG should not interfere with assays using the antibodies.

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