P8147
Platelet-Derived Growth Factor from human platelets
PDGF, lyophilized powder, suitable for cell culture
Synonyme(s) :
PDGF
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About This Item
Produits recommandés
Source biologique
human
Niveau de qualité
Pureté
≥95% (SDS-PAGE)
Forme
lyophilized powder
Puissance
0.5-10.0 ng/mL EC50
Qualité
endotoxin tested
Poids mol.
glycoprotein 28-31 kDa
Conditions de stockage
avoid repeated freeze/thaw cycles
Technique(s)
cell culture | mammalian: suitable
Impuretés
≤1 EU/μg
Numéro d'accès UniProt
Température de stockage
−20°C
Informations sur le gène
human ... PDGFA(5154) , PDGFB(5155)
Description générale
Platelet-derived growth factor (PDGF) is expressed as homo and heterodimers: PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD. The PDGF gene is highly conserved and is mapped to human chromosome 7p22.3. The active form of PDGF exists as disulfide-linked dimers. It consists of cystine-knot-fold growth factor domain, which is highly conserved, except for the N-terminal prodomain, which shows variation in length among the isoforms.
Application
PDGF is a chemoattractant for fibroblasts, smooth muscle cells, monocytes and neutrophils. Also, it acts on fibroblasts and other cells that initiate healing and tissue restoration.
Actions biochimiques/physiologiques
Platelet-derived growth factor (PDGF), not only functions as a growth factor, but also participates in chemotaxis for triggering cell movement. Elevated levels of placental PDGF is observed during the late first and third-trimester pregnancies. PDGF is associated with wound healing. PDGFRα (PDGF receptor α ) phosphorylation, mediated by PDGFA activates pathways associated with cancer. PDGFA has been indicated to induce growth and metastasis in human hepatocellular carcinoma. Vascular endothelial growth factor expression is regulated by PDGF-AA autocrine signaling and is known to promote tumor angiogenesis in vivo. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.
The principal mitogen found in mammalian serum, released from platelets during clot formation. Elicits mitogenesis of mesoderm-derived cells, increased extracellular matrix synthesis, and chemotaxis and activation of neutrophils, monocytes and fibroblasts. Mitogenic for dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. Appears to interact with transforming growth factor-β1 in accelerating wound healing. However, platelet-derived growth factor (PDGF) may also be pathogenic in arteriosclerosis and neoplasia.
Autres remarques
Isolated from human platelets, predominantly containing two covalently linked subunits (A and B).
Forme physique
Lyophilized from a 0.2-μm filtered solution in 40% acetonitrile plus 0.1% TFA
Remarque sur l'analyse
The mitogenic activity is tested in culture using Swiss 3T3 cells or NR6-3T3 fibroblasts.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Les clients ont également consulté
Angiogenesis during human extraembryonic development involves the spatiotemporal control of PDGF ligand and receptor gene expression
Holmgren L, et al.
Development, 113(3), 749-754 (1991)
Clinical Findings Associated with a De Novo Partial Trisomy 10p11. 22p15. 3 and Monosomy 7p22. 3 Detected by Chromosomal Microarray Analysis
Kohannim O, et al.
Case Reports in Genetics, 2011 (2011)
Shiki Fujino et al.
Molecular cancer research : MCR, 19(5), 812-822 (2021-02-14)
Recently developed molecularly targeted therapies such as EGFR inhibitors have notably improved the prognosis of patients with cancer. However, patients with KRAS and BRAF mutations do not currently benefit from these therapies. Here, we aimed to examine potential effects of
Overexpression of platelet-derived growth factor-BB increases tumor pericyte content via stromal-derived factor-alpha CXCR4 axis
Song N, et al.
Cancer Research, 69(15), 6057-6064 (2009)
Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas
Shikada Y, et al.
Cancer Research, 65(16), 7241-7248 (2005)
Articles
Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.
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