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P5459

Sigma-Aldrich

Protéase from Bacillus licheniformis

glycerol solution (50%)

Synonyme(s) :

Protéinase from Bacillus licheniformis, Subtilisine A

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About This Item

Numéro CAS:
9014-01-1
Numéro de classification (Commission des enzymes):
3.4.21.62 (BRENDA, IUBMB)
Numéro MDL:
MFCD00132092
Code UNSPSC :
12352204
eCl@ss :
32160410
Nomenclature NACRES :
NA.54

Source biologique

Bacillus licheniformis

Niveau de qualité

200

Forme

glycerol solution (50%)

Activité spécifique

≥7 units/mg protein (biuret)

Concentration

≥50 mg/mL

Solubilité

0.85% sodium chloride: soluble, clear (Diluted)
H2O: miscible

Température de stockage

2-8°C

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Description générale

The enzyme hydrolyzes gelatin optimally at 50 °C over the range of pH 8-10. It is stable between pH 6-10 at 50 °C. Commercially, it is used in laundry detergent formulations and also for the recovery of silver from photographic emulsions.

Application

Protease from Bacillus licheniformis has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.
Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Bacillus licheniformis, product P5459, has been used to hydrolyze muscle during the isolation of mitochondria from skeletal muscle.

Actions biochimiques/physiologiques

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.

Conditionnement

Package size based on protein content.

Définition de l'unité

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Pictogrammes

Health hazardCorrosion
GHS08,GHS05

Mention d'avertissement

Danger

Mentions de danger

H318,H334,H412

Classification des risques

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Rachel A Schlaak et al.
Frontiers in cardiovascular medicine, 7, 23-23 (2020-03-21)
Radiation therapy is received by over half of all cancer patients. However, radiation doses may be constricted due to normal tissue side effects. In thoracic cancers, including breast and lung cancers, cardiac radiation is a major concern in treatment planning.
H T Skiri et al.
Chemical senses, 29(3), 253-267 (2004-03-30)
The primary olfactory centre, the antennal lobe of Heliothis virescens moths, contains 62 glomeruli which process plant odour information and four male-specific glomeruli which form the macroglomerular complex, involved in processing information about pheromone and interspecific signals. Using calcium imaging
Karima Ait-Aissa et al.
Scientific reports, 9(1), 7623-7623 (2019-05-22)
Coronary artery disease (CAD) is a leading cause of death worldwide and frequently associated with mitochondrial dysfunction. Detailed understanding of abnormalities in mitochondrial function that occur in patients with CAD is lacking. We evaluated mitochondrial damage, energy production, and mitochondrial
Erin L Seifert et al.
The Journal of biological chemistry, 283(37), 25124-25131 (2008-07-17)
Uncoupling protein-3 (UCP3) is a mitochondrial inner membrane protein expressed most abundantly in skeletal muscle and to a lesser extent in heart and brown adipose tissue. Evidence supports a role for UCP3 in fatty acid oxidation (FAO); however, the underlying
H Ikemura et al.
The Journal of biological chemistry, 262(16), 7859-7864 (1987-06-05)
Subtilisin E, an alkaline serine protease of Bacillus subtilis 168, is first produced as a precursor, pre-pro-subtilisin, which consists of a signal peptide for protein secretion (pre-sequence) and a peptide extension of 77 amino acid residues (pro-sequence) between the signal
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Protocoles

Enzymatic Assay of Protease Using Casein As a Substrate

To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.

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