P4439
Phosphatase, Alkaline from porcine kidney
lyophilized powder, ≥100 DEA units/mg protein
Synonyme(s) :
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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About This Item
Produits recommandés
Forme
lyophilized powder
Niveau de qualité
Activité spécifique
≥100 DEA units/mg protein
Composition
Protein, ≥50%
Température de stockage
−20°C
Catégories apparentées
Application
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling.
Actions biochimiques/physiologiques
Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.
Conditionnement
Package size based on DEA units
Définition de l'unité
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.
Forme physique
Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0
Remarque sur l'analyse
Protein determined by biuret.
Inhibiteur
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Protocoles
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
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