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N2770

Sigma-Aldrich

p-Nitrophenyl phosphate

SIGMAFAST™, chromogenic, tablet

Synonyme(s) :

pNPP tablets

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About This Item

Numéro CE :
224-246-5
Numéro MDL:
MFCD00284586
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.83

product name

SIGMAFAST™ p-Nitrophenyl phosphate Tablets, tablet, To prepare 20 mL

Forme

tablet

Solubilité

water: 0.25 tablet/mL, clear, colorless

Température de stockage

−20°C

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Catégories apparentées

Application

Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each Sigma FAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.

Reconstitution

Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing pNPP.

Informations légales

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Phillipp Hartmann et al.
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Alcohol-associated liver disease (ALD) is an important cause of morbidity and mortality worldwide. The intestinal microbiota is involved in the development and progression of ALD; however, little is known about commensal fungi therein. We studied the dynamic changes of the
Yau-Hung Chen et al.
International journal of biological macromolecules, 126, 159-169 (2018-12-27)
To fulfill the properties of membrane for guided bone tissue regeneration, chitosan (CS) and calcium phosphates were blended to produce porous hybrid membranes by lyophilization. We synthesized three different calcium phosphates: calcium deficient hydroxyapatite (CDHA), biphasic calcium phosphate (BCP) and
G Gasperini et al.
Infection and immunity, 89(4) (2020-12-16)
Typhoid and paratyphoid fevers have a high incidence worldwide and coexist in many geographical areas, especially in low-middle-income countries (LMIC) in South and Southeast Asia. There is extensive consensus on the urgent need for better and affordable vaccines against systemic
Öykü Üzülmez et al.
Frontiers in plant science, 12, 723363-723363 (2021-10-22)
Peanut allergy is a potentially life-threatening disease that is mediated by allergen-specific immunoglobulin E (IgE) antibodies. The major peanut allergen Ara h 2, a 2S albumin seed storage protein, is one of the most dangerous and potent plant allergens. Ara
Fernando Diaz et al.
Virology, 513, 129-135 (2017-10-27)
Herpes simplex virus 2 (HSV-2) is the leading cause of genital herpes and increases the risk of HIV infection, but there is no effective vaccine. A replication-defective HSV-2 mutant virus, dl5-29, is effective in animal models and has been in
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Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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