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MSANGERG

Sigma-Aldrich

Sanger Arrayed Whole Genome Lentiviral CRISPR Library

Mouse, Glycerol Format

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About This Item

Code UNSPSC :
12352200

Niveau de qualité

200

Conditionnement

pkg of 50 μL (96-well plate)

Application(s)

CRISPR

Conditions d'expédition

dry ice

Température de stockage

−70°C

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Catégories apparentées

Description générale

Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery.

Application

Functional Genomics/Screening /Target Validation
Sanger Arrayed Whole Genome Lentiviral CRISPR Library has been used for Functional Genomics/Screening /Target Validation.

Caractéristiques et avantages

  • Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
  • Simplify the workflow with puromycin selection
  • Illuminate CRISPR-expressing cells with BFP
  • Flip the expression components into and out of the genome using transposase

Additional Features
  • Better, not bigger: Two optimized clones per human gene reduces the time, cost, and scale of screening experiments
  • Ready-to-screen: Clones are arrayed in a robotics-friendly 96-well format for high throughput screening; requires plasmid preparation to transfect cells or lentivirus preparation to transduce cells
  • Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here

Conditionnement

96-well plates

Composants

Bacteria glycerol stock of gRNA-only 3rd generation lenti-based plasmid vector. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones

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Forme physique

50 μl of bacterial glycerol stocks, 450×96-well plates

Autres remarques

This product is for R&D use only, not for drug, household, or other uses. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

Produits recommandés

Additional libraries are available:
Human, Glycerol Format
Human, Virus Format
Mouse, Virus Format

Informations légales

CRISPR Use License Agreement

Lentiviral License Agreement

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries
Emmanouil Metzakopian
Scientific Reports (2017)

Protocoles

Sanger Sequencing Steps & Method

Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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