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MAK317

Sigma-Aldrich

Sorbitol Dehydrogenase Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

endocrinological disorders, diabetes; gastrointestinal diseases

Température de stockage

−20°C

Catégories apparentées

Description générale

Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage. Thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy.
Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage; thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy.

Caractéristiques et avantages

Compatible with high-throughput handling systems.

Adéquation

Suitable for determining sorbitol dehydrogenase (SDH) activity in a number of biological samples such as plasma, serum, urine, tissue, and culture media.

Principe

Sorbitol dehydrogenase assay is a non-radioactive, colorimetric assay. It is based on the reduction of the tetrazolium salt MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) in a NADH-coupled enzymatic reaction. The reduced form of MTT exhibits an absorption maximum at 565 nm and the increase in absorbance at 565 nm is directly proportional to the enzyme activity. This assay is based on a kinetic reaction. Sorbitol dehydrogenase (SDH) activity can be determined in a number of biological samples (e.g., plasma, serum, urine, tissue, and culture media).

Pictogrammes

FlameCorrosionExclamation mark
GHS02,GHS05,GHS07

Mention d'avertissement

Warning

Mentions de danger

H226,H290,H315,H319,H336

Classification des risques

Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

75.2 °F - closed cup

Point d'éclair (°C)

24 °C - closed cup

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

The current state of serum biomarkers of hepatotoxicity.
Ozer J, et al.
Toxicology, 245(3), 194-205 (2008)
Pathophysiology of diabetic retinopathy.
Tarr J M, et al.
ISRN ophthalmology (2013)

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