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MAK561

Sigma-Aldrich

Succinate Dehydrogenase Assay Kit

sufficient for 100 colorimetric tests

Synonyme(s) :

SDH Activity Assay Kit

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About This Item

Code UNSPSC :
41116104
Nomenclature NACRES :
NA.28

Utilisation

sufficient for 100 colorimetric tests

Niveau de qualité

100

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

neurological disorders

Température de stockage

−20°C

Description générale

Succinate Dehydrogenase (SDH) is part of complex II found in the inner part of the mitochondrial membrane. SDH is part of the citric acid cycle (TCA) that catalyze the oxidation of succinate to fumarate. Electrons from succinate are transferred to FAD forming FADH2 which transfer electrons to CoQ. Mutations in SDH cause hereditary paraganglioma/phaeochromocytoma syndrome and a neurodegenerative disorder known as Leigh syndrome. The Succinate Dehydrogenase Assay kit provides a simple and sensitive procedure for measuring SDH activity in a variety of tissues, cells, and isolated mitochondria. SDH activity is determined by generating a product with absorbance at 600 nm proportional to the enzymatic activity present. One unit of SDH is the amount of enzyme that generates 1.0 μmole of DCIP per minute at pH 7.2 at 25 °C.

Application

Succinate Dehydrogenase Assay Kit has been used to measure the SDH enzyme activity in cell and tissue culture supernatants and in purified mitchondria.

Caractéristiques et avantages

  • Highly sensitive, simple and high-throughput assay for measuring Succinate Dehydrogenase (SDH) concentration.
  • Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
Click here to download a calculator excel file

Adéquation

Suitable for the detection of Succinate Dehydrogenase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Pictogrammes

Skull and crossbonesEnvironment
GHS06,GHS09

Mention d'avertissement

Danger

Mentions de danger

H302,H311 + H331,H411

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

188.6 °F

Point d'éclair (°C)

87 °C

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