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MAK246

Sigma-Aldrich

Neutrophil Elastase Activity Assay Kit (Fluorometric)

sufficient for 100 fluorometric tests

Synonyme(s) :

Elastase Enzyme Activity Fluorescent Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.25

Méthode de détection

fluorometric

Maladie(s) pertinente(s)

hematological disorder; pulmonary disorders

Température de stockage

−20°C

Description générale

Neutrophil Elastase (NE, EC 3.4.21.37, leukocyte elastase, ELANE, ELA2, elastase 2, neutrophil elaszym, or serine elastase) is a cytotoxic serine protease. It is stored in the azurophil granules of neutrophil granulocyte and is released following cell stimulation, e.g. by pathogens, immune complexes or chemotactic agents (PMA). Mutation in the gene ELA2 (neutrophil elastase) results in neutropenia, a hereditary disorder characterized by reduced neutrophil count in an individual.

Application


  • Relationship between airway inflammation and airflow limitation in elderly asthmatics.: This study investigates the connection between airway inflammation and airflow limitation in elderly asthmatic patients. It highlights the role of neutrophil elastase in airway inflammation, which was measured using the Neutrophil Elastase Activity Assay Kit (Fluorometric). The findings suggest that targeting neutrophil elastase activity could help in managing airflow limitation in elderly asthmatics, offering potential therapeutic applications for the assay kit (Soma et al., 2020).

Adéquation

Suitable to determine the activity of purified Neutrophil Elastase, detect the activity of Neutrophil Elastase in plasma and/or blood and screen/study/characterize chemotactic agents causing Neutrophil stimulation and release of Elastase.

Principe

The Neutrophil Elastase Activity Assay Kit utilizes the ability of NE to proteolytically cleave a synthetic substrate and release a fluorophore, AFC, which can be easily quantified by fluorescence. This assay kit is simple, rapid, and can detect as low as 1 ng of neutrophil elastase in a variety of samples.

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

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Certificats d'analyse (COA)

Lot/Batch Number
6M29K03830
7B26K03830
6G24K03830
5M13K03830
SLCC6368

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Neutrophil elastase in cyclic and severe congenital neutropenia.
Horwitz M S, et al.
Blood, 109(5), 1817-1824 (2007)
Sang-Il Han et al.
Bioresource technology, 302, 122791-122791 (2020-01-26)
The effect of co-cultivation of Porphyridium cruentum UTEX 161 with Pseudoalteromonas sp. MEBiC 03485 on P. cruentum growth and its sulfated polysaccharide (EPS) production were examined. The strain MEBiC 03485 had beneficial effects on P. cruentum growth, EPS production, and

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