324740

Elastase Substrate V, Fluorogenic

Elastase Substrate V, Fluorogenic, CAS 72252-90-5, is a sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (K_m = 140 µM).

• Synonyme(s) : Elastase Substrate V, Fluorogenic, MeOSuc-AAPV-AMC
• Formule empirique (notation de Hill): C₃₁H₄₁N₅O₉
• Numéro CAS: 72252-90-5
• Poids moléculaire : 627.69
• Numéro MDL: MFCD00077135
• Code UNSPSC : 12352200

Propriétés

• Niveau de qualité: 200
• Pureté: ≥95% (HPLC); ≥95% (TLC)
• Forme: lyophilized solid; powder
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated; protect from light
• Couleur: white to off-white
• Solubilité: ethanol: 1 mg/mL; DMF: 5 mg/mL; DMSO: 5 mg/mL
• Fluorescence: λ_ex 360-380 nm; λ_em 430-460 nm
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• InChI: 1S/C31H41N5O9/c1-16(2)27(34-20-9-10-21-17(3)14-26(39)45-23(21)15-20)30(42)35-29(41)22-8-7-13-36(22)31(43)19(5)33-28(40)18(4)32-24(37)11-12-25(38)44-6/h9-10,14-16,18-19,22,27,34H,7-8,11-13H2,1-6H3,(H,32,37)(H,33,40)(H,35,41,42)/t18-,19-,22-,27-/m0/s1
• Clé InChI: NHMQNKPYBPDCAY-NFHWZJRKSA-N

Description

Description générale

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte (K_m = 140 µM; K_cat/K_m = 120,000 s^-1 M^-1) and porcine pancreatic elastase. Not hydrolyzed by cathepsin G. Excitation max.: ~380 nm; emission max.: ~460 nm.

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (K_m = 140 µM; k_cat/K_m = 120,000 M^-1 s^-1). Not hydrolysed by cathepsin G.

Actions biochimiques/physiologiques

Cell permeable: no

Km = 140 µM; kcat/Km = 120,000 M⁻¹ s⁻¹as substrates for human leukocyte and porcine pancreatic elastase

Primary Target
Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Standard Handling (A)

Séquence

MeOSuc-Ala-Ala-Pro-Val-AMC

Reconstitution

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 months at -20°C.

Autres remarques

Barrett, A.J. 1981. Methods Enzymol. 80, 581.
Castillo, M.J., et al. 1979. Anal. Biochem.99, 53.
Nakajima, K., et al. 1979. J. Biol. Chem.254, 4027.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable