MAK161
Multidrug Resistance Assay Kit
(Fluorometric MDR Assay)
Synonyme(s) :
MDR Assay
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Utilisation
(Fluorometric MDR Assay)
Application(s)
pharmaceutical
Méthode de détection
fluorometric
Maladie(s) pertinente(s)
cancer
Température de stockage
−20°C
Informations sur le gène
human ... ABCB1(5243) , ABCC2(1244)
mouse ... ABCC2(12780)
rat ... abcc2(25303)
Catégories apparentées
Description générale
Acquired resistance to chemotherapy drugs, multidrug resistance or MDR, is a major contributor to treatment failure for many types of cancers. MDR is typically associated with the increased expression of two ATP-dependent drug efflux pumps, P-Glycoprotein (P-gp or MDR1) and the Multidrug Resistance-Associated Protein (MRP1). These pumps actively expel chemotherapeutic agents, typically hydrophobic amphipathic natural products, from the cytoplasm to exterior of the cell.
This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λex = 490/λem = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.
This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λex = 490/λem = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.
Caractéristiques et avantages
Compatible with high-throughput handling systems.
Adéquation
This kit is suitable for the screening of MDR pump inhibitors or for identifying cell lines with high MDR activity.
Principe
This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λEx = 490/λEm = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.
Code de la classe de stockage
10 - Combustible liquids
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Drug resistance in cancer: an overview.
Housman G, et al.
Cancer, 6(3), 1769-1792 (2014)
Non-coding polymorphisms in nucleotide binding domain 1 in ABCC1 gene associate with transcript level and survival of patients with breast cancer.
Kunicka T, et al.
PLoS ONE, 9(7), e101740-e101740 (2014)
Mechanisms and insights into drug resistance in cancer.
Zahreddine H and Katherine B
Frontiers in Pharmacology, 4, 28-28 (2013)
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