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MAK138

Sigma-Aldrich

Autophagy Assay Kit

sufficient for 200 fluorometric tests

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 200 fluorometric tests

Méthode de détection

fluorometric

Maladie(s) pertinente(s)

cancer; immunological diseases

Température de stockage

−20°C

Catégories apparentées

Description générale

Autophagy is an evolutionarily conserved degradation process that targets long-lived proteins, organelles, and other cytoplasmic components for degradation via the lysosomal pathway. Activation of the autophagy pathway is required for multiple cellular roles, including survival during starvation, the clearance of intracellular components, development, and immunity.

Application


  • Inhibitory Effect of Ursolic Acid on Proliferation and Migration of Renal Carcinoma Cells and Its Mechanism.: This study explores the inhibitory effects of ursolic acid on the proliferation and migration of renal carcinoma cells. The use of an autophagy assay kit was instrumental in demonstrating that ursolic acid induces autophagy, leading to decreased cell viability and migration. These findings highlight the potential of ursolic acid as a therapeutic agent in renal carcinoma through the modulation of autophagy (Lyu et al., 2022).

Adéquation

This kit is suitable for the detection of autophagy in mammalian cells by fluorescence microscopy and fluorescence microplate readers.

Principe

The Autophagy Assay kit provides a simple and direct procedure for measuring autophagy in a variety of cell types using a proprietary fluorescent autophagosome marker (λex = 333/λem = 518 nm).

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

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Autophagy in the pathogenesis of disease.
Levine B and Guido K
Cell, 132(1), 27-42 (2008)
Autophagy: machinery and regulation.
Yin Z, et al.
Microbial cell, 3(12), 588-588 (2016)
Ilenia Martinelli et al.
Redox biology, 40, 101866-101866 (2021-01-26)
Autophagy and apoptosis are powerful regulators of multiple facets of cellular metabolism and homeostasis. Here, we uncover that galanin, a pleiotropic peptide, regulates cardiac autophagy and deactivates apoptotic cell death through the Forkhead box protein O1 (FoxO1) pathway. In hypertrophied
Simin Nazarnezhad et al.
Neurotoxicology, 74, 196-202 (2019-07-20)
Diabetic neuropathy (DN) is the most common complication of diabetes mellitus. It is thought that neuronal cell death which is mainly due to reactive oxygen species (ROS) overproduction in the cells is responsible for most symptoms of this disorder. Nesfatin-1
Platelet rich plasma (PRP) induces chondroprotection via increasing autophagy, anti-inflammatory markers, and decreasing apoptosis in human osteoarthritic cartilage.
Moussa M, et al.
Experimental Cell Research, 352(1), 146-156 (2017)
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