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MAK082

Sigma-Aldrich

Aldehyde Dehydrogenase Activity Colorimetric Assay Kit

sufficient for 100 colorimetric tests

Synonyme(s) :

ALDH Activity Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric tests

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

cardiovascular diseases; cancer; hematological disorder

Température de stockage

−20°C

Description générale

Aldehyde dehydrogenases (ALDH) are a family of enzymes that catalyze the metabolism of exogenous and endogenous aldehydes, preventing the accumulation of potentially reactive and toxic aldehydes and their metabolites. In addition to their role in aldehyde metabolism, ALDH enzymes also play critical roles in other cellular processes such as cell proliferation, differentiation, and survival. Mutations in ALDH family members have been linked to numerous diseases such as heart disease, cataracts, and certain cancers. ALDH activity may also serve as a marker for certain stem cell populations including hematopoietic stem cells and certain cancer stem cells.

Application

Aldehyde Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the activity of aldehyde dehydrogenase.

Caractéristiques et avantages

Compatible with high-throughput handling systems.

Adéquation

Suitable for the measurement of aldehyde dehydrogenase (ALDH) in a variety of samples including tissue and cells

Principe

The Aldehyde Dehydrogenase Activity Colorimetric Assay kit provides a simple and direct procedure for measuring NAD-dependent ALDH activity in a variety of samples. ALDH activity is determined by a coupled enzyme assay in which acetaldehyde is oxidized by ALDH generating NADH, which reacts with a probe generating a colorimetric (450 nm) product proportional to the ALDH activity present. One unit of ALDH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at room temperature.

Pictogrammes

Health hazardCorrosion
GHS08,GHS05

Mention d'avertissement

Danger

Mentions de danger

H314,H350

Classification des risques

Carc. 1B - Eye Dam. 1 - Skin Corr. 1B

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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Certificats d'analyse (COA)

Lot/Batch Number
7L23K07310
7D06K07310
6K05K07310
6D28K07310
6J03K07310

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Aldehyde Dehydrogenases in Cellular Responses to Oxidative/electrophilic Stress
Surendra S, et al.
Free Radical Biology & Medicine, 56, 89-101 (2013)
Characterization of the CD49f+/CD44+/CD24- single-cell derived stem cell population in basal-like DCIS cells.
Duru N, et al.
Oncotarget, 7(30), 47511-47511 (2016)
Xiaotian Xu et al.
Aging, 14(11), 4858-4873 (2022-06-10)
The roles of asparagine-linked glycosylation (ALG) members in tumorigenic process have been widely explored. However, their effects in colorectal cancer progression are still confusing. Here, we screened 12 ALGs' expression through online datasets and found that ALG10 was mostly upregulated
A Novel Long Non-Coding RNA lnc030 Maintains Breast Cancer Stem Cell Stemness by Stabilizing SQLE mRNA and Increasing Cholesterol Synthesis.
Yilu Qin et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9(24), e2204046-e2204046 (2022-08-26)
Yilu Qin et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 8(2), 2002232-2002232 (2021-01-30)
Cancer stem cells (CSCs) are considered the roots of cancer metastasis and recurrence (CSCs), due in part to their self-renewal and therapy resistance properties. However, the underlying mechanisms for the regulation of CSC stemness are poorly understood. Recently, increasing evidence
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Articles

Cancer Stem Cell Detection Utilizing a Red-shifted Fluorescent Live Cell Probe for Aldehyde Dehydrogenase (ALDH), AldeRed™ 588-A.

AldeRed™ 588-A is a red fluorescent live cell probe that detects ALDH activity used to identify cancer stem cells and progenitor cells in culture. Cancer stem cells (CSCs) are subpopulations of cancer cells that can self-renew, generate diverse cells in the tumor mass, and sustain tumorigenesis. Some researchers believe that cancer arises from cancer stem cells that originate as a result of mutational hits on normal stem cells.

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