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MAK327

Sigma-Aldrich

Aldehyde Dehydrogenase Inhibitor Screening Kit

sufficient for 100 colorimetric tests

Synonyme(s) :

ALDH Inhibitor Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric tests

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

cancer

Température de stockage

−20°C

Description générale

Aldehyde Dehydrogenases (ALDHs) are a superfamily of oxidoreductases which catalyze the conversion of aldehydes to carboxylic acids. ALDH is crucial in the metabolism of alcohol as alcohol dehydrogenase breaks down ethanol to acetaldehyde. Acetaldehyde, which is toxic to the body, is in turn broken down by ALDH to acetic acid. Imbalances of aldehyde dehydrogenase have been linked to both alcoholism and alcohol sensitivity in people. Inhibitors of the enzyme have been used in cases to treat alcoholism in patients. Cancer stem cell populations also display a heightened activity of ALDH, making ALDH inhibition a promising anti-cancer therapy approach.

Application

The Aldehyde Dehydrogenase Inhibitor Screening Kit may be used for:
  • Alcohol Metabolism Research
  • Cancer Research
  • Drug Discovery

Caractéristiques et avantages

  • High-Throughput Compatibility: The kit seamlessly integrates into high-throughput handling systems, ensuring efficiency and accuracy.
  • Efficient Inhibitor Screening: Screen for ALDH inhibitors with as little as 5 µL of test compound, facilitated by a streamlined procedure requiring minimal effort.

Adéquation

Suitable for inhibitor screening and evaluation of ALDH inhibitors.

Principe

The Aldehyde Dehydrogenase Inhibitor Screening Kit is based on the enzymatic conversion of acetaldehyde to acetic acid and NADH by ALDH. The formed NADH in turn reduces a formazan reagent into a colored product the absorbance of which, measured at 565 nm, is proportional to the enzyme activity in the reaction. The percent inhibition of a test compound can be determined by comparing the activity of ALDH treated with a test compound to the activity of untreated ALDH.

Autres remarques

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Pictogrammes

FlameHealth hazardExclamation mark
GHS02,GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H226,H312 + H332,H319,H360D

Classification des risques

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

Code de la classe de stockage

3 - Flammable liquids

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