Accéder au contenu
Merck
Toutes les photos(1)

Principaux documents

Voir toute la documentation

M9004

Sigma-Aldrich

Malic Dehydrogenase from bovine heart

ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)

Synonyme(s) :

L-Malate:NAD+ oxidoreductase, MDH

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro de classification (Commission des enzymes):
1.1.1.37 (BRENDA, IUBMB)
Numéro CE :
232-622-5
Numéro MDL:
MFCD00131567
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

bovine heart

Niveau de qualité

200

Forme

ammonium sulfate suspension

Activité spécifique

2000-4000 units/mg protein (modified Warburg-Christian)

Poids mol.

35 kDa

Impuretés

≤0.01% Glutamic-Oxalacetic Transaminase
≤0.01% Glutamic-Pyruvic Transaminase

Température de stockage

2-8°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed.
Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Application

Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological processes. It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas.

Définition de l'unité

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.

Forme physique

Suspension in 3 M (NH4)2SO4 - 0.01 M KH2PO4 solution, pH 7.3

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
SLCH6610
SLCB1651
098K7680V
098K7680
058H78041

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Enika Nagababu et al.
Methods in molecular biology (Clifton, N.J.), 610, 165-180 (2009-12-17)
Reactive oxygen species are implicated in many human diseases and aging process. Much of the evidence is based on experimental data indicating increasing rates of lipid peroxidation in disease states and the ameliorating effects of antioxidants. It is becoming increasingly
E M Gregory
The Journal of biological chemistry, 250(14), 5470-5474 (1975-07-25)
Bovine mitochondrial malate dehydrogenase (EC 1.1.1.37) was inactivated by the specific modifications of a single histidine residue upon reaction with iodoacetamide. NADH protected against this loss of activity and reaction with the histidine residue, suggesting that the histidine is at
Eisaku Hokazono et al.
Clinica chimica acta; international journal of clinical chemistry, 412(15-16), 1436-1440 (2011-05-10)
In human serum, as for phospholipids not containing choline, phosphatidylethanolamine (PE) exists approximately 5% in a whole phospholipid. PE is well known as one of the main components of biological membranes, and also plays important roles that contribute to apoptosis
Flow injection system for on-line monitoring of fumaric acid in biological processes
Rhee, J.
Analytica Chimica Acta, 499, 71-80 (2003)
Isoelectric points and molecular weights of proteins: a new table.
Righetti, P.G., and Tudor, G.
Journal of Chromatography A, 220, 115-194 (1981)
Afficher tous les articles scientifiques apparentés

Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocoles

Enzymatic Assay of Malic Dehydrogenase (EC 1.1.1.37)

Enzymatic Assay of Malic Dehydrogenase (EC 1.1.1.37)

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique