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M4159

Methylene Blue hydrate

Name: Methylene Blue hydrate
Brand: SIGMA

suitable for nucleic acid staining, BioReagent

Synonyme(s) :

3,7-Bis(dimethylamino)phenothiazin-5-ium chloride xhydrate, Methylene Blue monohydrate, Methylene blue, Nucleic acid staining reagent

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About This Item

Formule empirique (notation de Hill):

C₁₆H₁₈ClN₃S · xH₂O

Numéro CAS:

122965-43-9

Poids moléculaire :

319.85 (anhydrous basis)

Numéro Beilstein :

3599847

Numéro CE :

200-515-2

Numéro MDL:

MFCD00150006

Code UNSPSC :

12171500

ID de substance PubChem :

57654386

Nomenclature NACRES :

NA.52

Qualité

for molecular biology

Gamme de produits

BioReagent

Forme

powder

Technique(s)

nucleic acid detection: suitable

Solubilité

water: 1 mg/mL (dark blue)

Adéquation

suitable for nucleic acid staining

Température de stockage

room temp

Chaîne SMILES

O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1

Clé InChI

WQVSELLRAGBDLX-UHFFFAOYSA-M

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Description générale

Methylene blue is a heterocyclic aromatic chemical, appearing as a dark green solid powder, changing to blue in aqueous solution. It is used as a stain in many different histology procedures, as well as in molecular biology laboratories.

Application

Methylene Blue hydrate is suitable for use as a nucleic acid stain for agarose and polyacrylamide gel electrophoresis (PAGE). It was used to stain 4T1 tumor cells to study metastasis of tumor cells using clonogenic assay. Methylene blue hydrate crystals were encapsulated in apoferritin nanocages for photodynamic therapy against tumor cells.

Caractéristiques et avantages

Less hazardous than staining with ethidium bromide
Viewable in visible light
Fast and easy to use

Produit(s) apparenté(s)

Réf. du produit

Description

Tarif

Mention d'avertissement

Warning

Mentions de danger

H302

Classification des risques

Acute Tox. 4 Oral

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Les clients ont également consulté

Slide 1 of 2

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USP

1428008

Methylene blue

Sigma-Aldrich

457250

Methylene Blue

Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.

A C Peacock et al.

Biochemistry, 6(6), 1818-1827 (1967-06-01)

Young-Sharp, D. and Kumar, R.

Technique, 1, 183-183 (1989)

Hydrogen bond acceptors and additional cationic charges in methylene blue derivatives: photophysics and antimicrobial efficiency.

Ariane Felgenträger et al.

BioMed research international, 2013, 482167-482167 (2013-03-20)

Photodynamic inactivation of bacteria (PIB) by efficient singlet oxygen photosensitizers might be a beneficial alternative to antibiotics in the struggle against multiresistant bacteria. Phenothiazinium dyes belong to the most prominent classes of such sensitizers due to their intense absorption in

Phenotypic identification of the redox dye methylene blue as an antagonist of heat shock response gene expression in metastatic melanoma cells.

Angela L Davis et al.

International journal of molecular sciences, 14(2), 4185-4202 (2013-02-23)

Repurposing approved and abandoned non-oncological drugs is an alternative developmental strategy for the identification of anticancer therapeutics that has recently attracted considerable attention. Due to the essential role of the cellular heat shock response in cytoprotection through the maintenance of

Cellular uptake and photodynamic activity of protein nanocages containing methylene blue photosensitizing drug.

Fei Yan et al.

Photochemistry and photobiology, 86(3), 662-666 (2010-02-06)

This study reports that photosensitizers encapsulated in supramolecular protein cages can be internalized by tumor cells and can deliver singlet oxygen intracellularly for photodynamic therapy (PDT). As an alternative to other polymeric and/or inorganic nanocarriers and nanoconjugates, which may also

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Protocoles

Methylene Blue - Molecular Biology Reagent

Suitable for use in DNA and RNA staining.

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